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EC number: 219-616-8 | CAS number: 2481-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Auto flammability
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- Oxidation reduction potential
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- Additional physico-chemical properties of nanomaterials
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- Endpoint summary
- Stability
- Biodegradation
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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- Acute Toxicity
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- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Experimental study on genetic toxicity in vitro was conducted by Vincent F. Simmon (J Natl Cancer Inst ,1979) to determine the mutagenic nature of target substance4-(Diethylamino) azobenzene (2481-94-9). Genetic toxicity in vitro for 4-(Diethylamino) azobenzene was assessed for its possible mutagenic potential .For this purpose Salmonella-microsome assay was performed on Salmonella typhimurium strainTA1535, TA1537 and TA1538,using test substance concentration of 0-1000µg/plate (4.44µmol/plate).The test substance was exposed to the Salmonella typhimurium strainTA1535, TA1537 and TA1538 in the presence and absence of metabolic activation system.S9 mix, Adult male Sprague-Dawley rats (200- 250 g) pre-treated with Aroclor 1254 (500 mg/kg) were used to obtain liver for the metabolic activation system. The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity.Each experiment included solvent controls as well as known direct-acting mutagens and a mutagen that required metabolic activation. No mutagenic effects were observed during the study. Therefore4-(Diethylamino) azobenzene was considered to be non mutagenic in Salmonella typhimurium strainTA1535, TA1537 and TA1538 by AMES assay. Hence it cannot be classified as gene mutant in vitro.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication .
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of 4-(Diethylamino)azobenzene in Salmonella typhimurium strain TA1535, TA1537 and TA1538 by Salmonella-microsome assay.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- - Name of test material: 4-(Diethylamino)azobenzene
- Molecular formula: C16H19N3
- Molecular weight: 253.347 g/mol
- Smiles notation: c1(N(CC)CC)ccc(\N=N\c2ccccc2)cc1
- InChl: 1S/C16H19N3/c1-3-19(4-2)16-12-10-15(11-13-16)18-17-14-8-6-5-7-9-14/h5-13H,3-4H2,1-2H3/b18-17+
- Substance type: Organic
- Physical state: Solid - Target gene:
- Histidine
- Species / strain / cell type:
- S. typhimurium, other: strain TA1535, TA1537 and TA1538
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- other: uvrB-deficient (to reduce the repair ofchemically induced lesions in the DNA) and are rfa (to prevent the synthesis of a normal lipopolysaccheride.
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix, Adult male Sprague-Dawley rats (200- 250 g) pretreated with Aroclor 1254 (500 mg/kg) were used to obtain liver for the metabolic activation system
- Test concentrations with justification for top dose:
- 0-1000µg/plate (4.44µ/mol)
- Vehicle / solvent:
- Yes ,but not specified .
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: detailed data not available.
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 24 hour
Other: The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity. - Rationale for test conditions:
- No data available.
- Evaluation criteria:
- The numbers of histidine-independent revertants for each S. typhimurium strain were taken from the linear portion of dose-response curves after the background was subtracted. The range of spontaneous revertants was 25-55 for TA1535 and 7-25 for TA1537. A positive response was defined as a reproducible, dose-related increase in the
- Statistics:
- Not specified.
- Key result
- Species / strain:
- S. typhimurium, other: strain TA1535, TA1537 and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Not specified.
- Remarks on result:
- other: No mutagenic effect were observed.
- Conclusions:
- 4-(Diethylamino) azobenzene was evaluated for its mutagenic potential in Salmonella typhimurium strain TA1535, TA1537 and TA1538 by AMES assay.The test result was considered to be negative in the presece and absence of S9.
- Executive summary:
Genetic toxicity in vitro for 4-(Diethylamino) azobenzene was assessed for its possible mutagenic potential .For this purpose Salmonella-microsome assay was performed on Salmonella typhimurium strainTA1535, TA1537 and TA1538,using test substance concentration of 0-1000µg/plate (4.44µmol/plate).The test substance was exposed to the Salmonella typhimurium strainTA1535, TA1537 and TA1538 in the presence and absence of metabolic activation system.S9 mix, Adult male Sprague-Dawley rats (200- 250 g) pre-treated with Aroclor 1254 (500 mg/kg) were used to obtain liver for the metabolic activation system. The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity.Each experiment included solvent controls as well as known direct-acting mutagens and a mutagen that required metabolic activation. No mutagenic effects were observed during the study. Therefore4-(Diethylamino) azobenzene was considered to be non mutagenic in Salmonella typhimurium strainTA1535, TA1537 and TA1538 by AMES assay. Hence it cannot be classified as gene mutant in vitro.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Genotoxicity In-vitro
Various publications were reviewed to determine the mutagenic nature of 4-(Diethylamino) azobenzene (2481-94-9). The studies are as mentioned below:
Experimental study on genetic toxicity in vitro was conducted by Vincent F. Simmon (J Natl Cancer Inst ,1979) to determine the mutagenic nature of target substance4-(Diethylamino) azobenzene (2481-94-9). Genetic toxicity in vitro for 4-(Diethylamino) azobenzene was assessed for its possible mutagenic potential .For this purpose Salmonella-microsome assay was performed on Salmonella typhimurium strainTA1535, TA1537 and TA1538,using test substance concentration of 0-1000µg/plate (4.44µmol/plate).The test substance was exposed to the Salmonella typhimurium strainTA1535, TA1537 and TA1538 in the presence and absence of metabolic activation system.S9 mix, Adult male Sprague-Dawley rats (200- 250 g) pre-treated with Aroclor 1254 (500 mg/kg) were used to obtain liver for the metabolic activation system. The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity.Each experiment included solvent controls as well as known direct-acting mutagens and a mutagen that required metabolic activation. No mutagenic effects were observed during the study. Therefore4-(Diethylamino) azobenzene was considered to be non mutagenic in Salmonella typhimurium strainTA1535, TA1537 and TA1538 by AMES assay. Hence it cannot be classified as gene mutant in vitro.
Supporting Gene mutation toxicity study was performed by Yen-Ling Cheunget al.( CARCINOGENESIS , 1994) to determine the mutagenic nature of 4-(Diethylamino) azobenzene (2481-94-9) . Genetic toxicity study in vitro for 4-(Diethylamino) azobenzene (2481-94-9) was assessed for its possible mutagenic potential. For this purpose AMES assay was performed in S. typhimurium strains TA98, using test substance concentration of 0-100 µg/PLATE. A preincubation step was incorporated for 1h at 37°C in a shaking water bath. The test substance was exposed to salmonella typhimurium in the presence of metabolic activation. RAT, LIVER, MICROSOMES, AROCLOR 1254 and RAT, LIVER, MICROSOMES, 4-Diethylaminoazobenzene were used as Metabolic activation system. Evaluation was done considering a dose dependent increase in the number of histidine revertants/plate. Statistical analysis was carried out to observe the histidine revertants/plate. No mutagenic effects were observed during the study period in S. typhimurium strains TA98 in the presence of metabolic activation system. Therefore 4-(Diethylamino) azobenzene was considered to be non mutagenic in Ames test .Hence it cannot be classified as gene mutant in vitro.
Another supporting gene mutation toxicity study was performed by Joyce McCann et al (Food and Chemical Toxicology, 2002) to determine the mutagenic nature of4-(Diethylamino) azobenzene (2481-94-9). Genetic toxicity study in vitro for 4-(Diethylamino)azobenzene (2481-94-9)was assessed for its possible mutagenic potential. For this purpose AMES assay was performed in S. typhimurium strains TA98, using test substance concentration of <0.03µ mol /plate. A standard Salmonella plate test was performed. The test substance was exposed to salmonella typhimurium in the presence and absence of S9metabolic activation. S9 (RAT, LIVER, MICROSOMES, AROCLOR 1254) was used as Metabolic activation system. Evaluation was done considering a dose dependent increase in the number of histidine revertants/plate. Statistical analysis was carried out to observe the histidine revertants/plate. No mutagenic effects were observed during the study period in S. typhimurium strains TA98 in the presence and absence of metabolic activation system. Therefore 4-(Diethylamino) azobenzene was considered to be non mutagenic in Ames test .Hence it cannot be classified as gene mutant in vitro.
Based on the data available for the target chemical, it is concluded that 4-(Diethylamino) azobenzene (2481-94-9) does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
Justification for classification or non-classification
Thus based on the above annotation and CLP criteria for the target chemical, it is concluded that 4-(Diethylamino) azobenzene (2481-94-9) does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
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