N,N-diethyl-p-(phenylazo)aniline

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 12.80 mg/l when  4-(Diethylamino) azo -benzene exposed to Daphnia magna for 48hrs.

Toxicity to aquatic algae and cyanobacteria:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 11.092mg/l when  4-(Diethylamino)azobenzene exposed to Desmodesmus subspicatus for 48hrs.

Additional information

Summarized result of the toxicity of 4-(Diethylamino)azobenzene (2481 -94 -9) on the growth and other activity of aquatic invertebrates and algae are as follows:

Short term toxicity to aquatic invertebrates:

Based on the various experimental data and prediction data for the target chemical and read across chemicals have been reviewed to determine the toxic nature of target chemical 4-(Diethylamino)azobenzene (2481 -94 -9). The studies are as mention below:

In the first weight of evidence study for 4-(Diethylamino)azobenzene (2481 -94 -9), Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 12.80 mg/l when  4-(Diethylamino) azo -benzene exposed to Daphnia magna for 48hrs.

Similarly in the second weight of evidence study for RA chemical (86 -30 -6), j-check. Acute Immobilization Test of N-Nitrosodiphenylamine to Daphnia magna. Test was performed in the static system in which different concentration 3.00, 5.40, 9.30, 18.0, 30.0 mg/L was taken. Nominal values were adopted for calculation of effect concentration, because all measurement concentrations of test Substance which were measured at the start of exposure and after 48 hours were within ±20 % of nominal values.). After 24 hrs, Median immobilization concentration (EiC50) was 13.5 mg/L (95 % CL: 9.30 – 18.0 mg/L), No observed effect concentration (NOECi): 9.30 mg/L. And Lowest concentration of 100 % inhibition was 30.0 mg/L. But after 48 hrs. Exposure no observed effect concentration (NOECi) was 3.00 mg/L. And the lowest concentration of 100 % inhibition: 18.0 mg/L. Based on the immobilization of daphnia magna, due to the N-Nitrosodiphenylamine, the EC50 was 10.1 mg/l

Similarly in the third weight of evidence study for the RA chemical (140 -56 -7) ecotox, Study of short term toxicity of N-[[4-(Dimethylamino) phenyl]imino] sulfamic acid sodium salt on the growth of invertebrates. The study was carried out in the static system for 24 hrs.Based on the mortality of Gammarus fasciatus due to the N-[[4-(Dimethylamino)phenyl]imino]sulfamic acid sodium salt dye to the 24 hrs exposure the LC50 was 18 mg/l. Based on the LC50, N-[[4-(Dimethylamino) phenyl]imino] sulfamic acid sodium saltchemical was toxic and can be consider to be not classified as per the CLP classification criteria.

Based on the various experimental data and prediction data for the target chemical and read across chemicals, 4-(Diethylamino)azobenzene (2481 -94 -9) was toxic and can be consider to be toxic and can be classified in aquatic chronic 3 category.

Toxicity to aquatic algae and cyanobacteria:

Based on the various experimental data and prediction data for the target chemical and read across chemicals have been reviewed to determine the toxic nature of target chemical 4-(Diethylamino)azobenzene (2481 -94 -9). The studies are as mention below:

In the first weight of evidence study for 4-(Diethylamino)azobenzene (2481 -94 -9), Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 11.092mg/l when  4-(Diethylamino)azobenzene exposed to Desmodesmus subspicatus for 48hrs.

Similarly in the second weight of evidence study for the RA chemical (119 -64 -2) tetralin, from OECD SIDS report and IUCLID dataset 2000.Toxicity of tetralin on the growth of freshwater algae Scenedesmus subspicatus for 72hrs was syudied. Test was performed in Erlenmeyer flasks in static system. 1 g test substance/l was stirred in deionized water for 18 hours and filtered. The resulting concentration was 63 mg/l. The maximum concentration tested was 17 mg/l. No vehicle or solvent was used. Toxicity was checked in the interval of 24 hrs, at Six different concentration 1.20; 2.0; 3.6; 6.0; 10.0; 17.0 mg/l (nominal) and 0 mg/l for control.Based on the growth rate of Scenedesmus subspicatus (Algae) due to the 1,2,3,4-Tetrahydronaphthalene the EC10, EC50, EC90 was 5.3 mg/l, 11 mg/l and 25 mg/l. But on the basis of of biomass the EC10, EC50, EC90 was 3.8 mg/l, 7mg/l, 13mg/l. and the NOEC was 3.6mg/l. Based on the EC50 (11 mg/l) growth rate of Scenedesmus subspicatus (Algae) due to the 1, 2, 3, 4-Tetrahydronaphthalene, chemical was toxic and can be consider in chronic category 3 as per the CLP classification criteria.

Based on the various experimental data and prediction data for the target chemical and read across chemicals 1, 2, 3, 4-Tetrahydronaphthalene, chemical was toxic to aquatic invertebrates and also algae and can be consider in chronic category 3 as per the CLP classification criteria.