Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-616-8 | CAS number: 2481-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication .
Data source
Reference
- Reference Type:
- publication
- Title:
- In Vitro Mutagenicity Assays of Chemical Carcinogens and Related Compounds with Salmonella typhimurium
- Author:
- Vincent F. Simmon
- Year:
- 1 979
- Bibliographic source:
- J Natl Cancer Inst (1979) 62 (4): 893-899
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of 4-(Diethylamino)azobenzene in Salmonella typhimurium strain TA1535, TA1537 and TA1538 by Salmonella-microsome assay.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N-diethyl-p-(phenylazo)aniline
- EC Number:
- 219-616-8
- EC Name:
- N,N-diethyl-p-(phenylazo)aniline
- Cas Number:
- 2481-94-9
- Molecular formula:
- C16H19N3
- IUPAC Name:
- N,N-diethyl-p-(phenylazo)aniline
- Test material form:
- solid
- Details on test material:
- - Name of test material: 4-(Diethylamino)azobenzene; N,N-diethyl-p-(phenylazo)aniline
- Molecular formula: C16H19N3
- Molecular weight: 253.347 g/mol
- Smiles notation: c1(N(CC)CC)ccc(\N=N\c2ccccc2)cc1
- InChl: 1S/C16H19N3/c1-3-19(4-2)16-12-10-15(11-13-16)18-17-14-8-6-5-7-9-14/h5-13H,3-4H2,1-2H3/b18-17+
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: 4-(Diethylamino)azobenzene
- Molecular formula: C16H19N3
- Molecular weight: 253.347 g/mol
- Smiles notation: c1(N(CC)CC)ccc(\N=N\c2ccccc2)cc1
- InChl: 1S/C16H19N3/c1-3-19(4-2)16-12-10-15(11-13-16)18-17-14-8-6-5-7-9-14/h5-13H,3-4H2,1-2H3/b18-17+
- Substance type: Organic
- Physical state: Solid
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: strain TA1535, TA1537 and TA1538
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- other: uvrB-deficient (to reduce the repair ofchemically induced lesions in the DNA) and are rfa (to prevent the synthesis of a normal lipopolysaccheride.
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix, Adult male Sprague-Dawley rats (200- 250 g) pretreated with Aroclor 1254 (500 mg/kg) were used to obtain liver for the metabolic activation system
- Test concentrations with justification for top dose:
- 0-1000µg/plate (4.44µ/mol)
- Vehicle / solvent:
- Yes ,but not specified .
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: detailed data not available.
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 24 hour
Other: The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity. - Rationale for test conditions:
- No data available.
- Evaluation criteria:
- The numbers of histidine-independent revertants for each S. typhimurium strain were taken from the linear portion of dose-response curves after the background was subtracted. The range of spontaneous revertants was 25-55 for TA1535 and 7-25 for TA1537. A positive response was defined as a reproducible, dose-related increase in the
- Statistics:
- Not specified.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: strain TA1535, TA1537 and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- Not specified.
- Remarks on result:
- other: No mutagenic effect were observed.
Applicant's summary and conclusion
- Conclusions:
- 4-(Diethylamino) azobenzene was evaluated for its mutagenic potential in Salmonella typhimurium strain TA1535, TA1537 and TA1538 by AMES assay.The test result was considered to be negative in the presece and absence of S9.
- Executive summary:
Genetic toxicity in vitro for 4-(Diethylamino) azobenzene was assessed for its possible mutagenic potential .For this purpose Salmonella-microsome assay was performed on Salmonella typhimurium strainTA1535, TA1537 and TA1538,using test substance concentration of 0-1000µg/plate (4.44µmol/plate).The test substance was exposed to the Salmonella typhimurium strainTA1535, TA1537 and TA1538 in the presence and absence of metabolic activation system.S9 mix, Adult male Sprague-Dawley rats (200- 250 g) pre-treated with Aroclor 1254 (500 mg/kg) were used to obtain liver for the metabolic activation system. The strains were checked periodically for relevant genetic markers and for rfa by crystal violet sensitivity.Each experiment included solvent controls as well as known direct-acting mutagens and a mutagen that required metabolic activation. No mutagenic effects were observed during the study. Therefore4-(Diethylamino) azobenzene was considered to be non mutagenic in Salmonella typhimurium strainTA1535, TA1537 and TA1538 by AMES assay. Hence it cannot be classified as gene mutant in vitro.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
