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EC number: 219-616-8 | CAS number: 2481-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- (Q)SAR
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification
- Justification for type of information:
- Data is from OECD QSAR toolbox version 3.3 and the QMRAF report has been attached.
- Qualifier:
- according to guideline
- Guideline:
- other: Estimated data
- Version / remarks:
- No data available
- Deviations:
- not specified
- Principles of method if other than guideline:
- Prediction was done using the OECD QSAR toolbox version 3.3.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: 4-(Diethylamino)azobenzene
- Molecular formula: C16H19N3
- Molecular weight: 253.347 g/mol
- Smiles notation: c1(N(CC)CC)ccc(\N=N\c2ccccc2)cc1
- InChl: 1S/C16H19N3/c1-3-19(4-2)16-12-10-15(11-13-16)18-17-14-8-6-5-7-9-14/h5-13H,3-4H2,1-2H3/b18-17+
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Details on sampling:
- No data available
- Vehicle:
- not specified
- Details on test solutions:
- No data available
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- No data available
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- No data available
- Post exposure observation period:
- No data available
- Hardness:
- No data available
- Test temperature:
- 24±2°C
- pH:
- 7.9
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Conductivity:
- No data available
- Nominal and measured concentrations:
- No data available
- Details on test conditions:
- No data available
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12.801 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: According to predicted data chemical was toxic
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC50 value was estimated to be 12.80 mg/l when 4-(Diethylamino)azobenzene exposed to Daphnia magna for 48hrs.
- Executive summary:
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 12.80 mg/l when 4-(Diethylamino) azo -benzene exposed to Daphnia magna for 48hrs.
Reference
The
prediction was based on dataset comprised from the following
descriptors: EC50
Estimation method: Takes average value from the 6 nearest neighbours
Domain logical expression:Result: In Domain
(((((((((("a"
or "b" or "c" or "d" )
and ("e"
and (
not "f")
)
)
and "g" )
and "h" )
and "i" )
and "j" )
and ("k"
and (
not "l")
)
)
and ("m"
and (
not "n")
)
)
and ("o"
and (
not "p")
)
)
and ("q"
and "r" )
)
Domain
logical expression index: "a"
Referential
boundary: The
target chemical should be classified as Aromatic amine OR Aryl OR Azo by
Organic Functional groups ONLY
Domain
logical expression index: "b"
Referential
boundary: The
target chemical should be classified as Aromatic amine OR Aryl OR Azo OR
Overlapping groups by Organic Functional groups (nested) ONLY
Domain
logical expression index: "c"
Referential
boundary: The
target chemical should be classified as Aliphatic Carbon [CH] OR
Aliphatic Carbon [-CH2-] OR Aliphatic Carbon [-CH3] OR Aliphatic
Nitrogen, one aromatic attach [-N] OR Amino, aliphatic attach [-N<] OR
Aromatic Carbon [C] OR Azo [-N=N-] OR Olefinic carbon [=CH- or =C<] by
Organic functional groups (US EPA) ONLY
Domain
logical expression index: "d"
Referential
boundary: The
target chemical should be classified as Amine OR Aromatic compound OR
Azo compound OR Tertiary amine OR Tertiary mixed amine by Organic
functional groups, Norbert Haider (checkmol) ONLY
Domain
logical expression index: "e"
Referential
boundary: The
target chemical should be classified as Non binder, without OH or NH2
group by Estrogen Receptor Binding
Domain
logical expression index: "f"
Referential
boundary: The
target chemical should be classified as Non binder, impaired OH or NH2
group OR Non binder, MW>500 OR Non binder, non cyclic structure OR Weak
binder, NH2 group by Estrogen Receptor Binding
Domain
logical expression index: "g"
Referential
boundary: The
target chemical should be classified as High (Class III) by Toxic hazard
classification by Cramer (with extensions) ONLY
Domain
logical expression index: "h"
Referential
boundary: The
target chemical should be classified as Aromatic amine AND Aryl AND Azo
by Organic Functional groups ONLY
Domain
logical expression index: "i"
Referential
boundary: The
target chemical should be classified as Aromatic amine AND Aryl AND Azo
by Organic Functional groups ONLY
Domain
logical expression index: "j"
Referential
boundary: The
target chemical should be classified as Amine AND Aromatic compound AND
Azo compound AND Tertiary amine AND Tertiary mixed amine by Organic
functional groups, Norbert Haider (checkmol) ONLY
Domain
logical expression index: "k"
Referential
boundary: The
target chemical should be classified as Not categorized by Repeated dose
(HESS)
Domain
logical expression index: "l"
Referential
boundary: The
target chemical should be classified as Tamoxifen (Hepatotoxicity) Alert
by Repeated dose (HESS)
Domain
logical expression index: "m"
Referential
boundary: The
target chemical should be classified as Aromatic Amine Type Compounds
AND Arylazo Type Compounds by Oncologic Primary Classification
Domain
logical expression index: "n"
Referential
boundary: The
target chemical should be classified as Polycyclic Aromatic Hydrocarbons
- Homocyclic OR Siloxane Type Compounds by Oncologic Primary
Classification
Domain
logical expression index: "o"
Referential
boundary: The
target chemical should be classified as Not Related to an Existing
ECOSAR Class by Aquatic toxicity classification by ECOSAR
Domain
logical expression index: "p"
Referential
boundary: The
target chemical should be classified as Pyrazoles/Pyrroles OR Triazines,
Aromatic OR Vinyl/Allyl Ethers by Aquatic toxicity classification by
ECOSAR
Domain
logical expression index: "q"
Parametric
boundary:The
target chemical should have a value of log Kow which is >= 4.15
Domain
logical expression index: "r"
Parametric
boundary:The
target chemical should have a value of log Kow which is <= 6.6
Description of key information
Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 12.80 mg/l when 4-(Diethylamino) azo -benzene exposed to Daphnia magna for 48hrs.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 12.8 mg/L
Additional information
Based on the various experimental data and prediction data for the target chemical and read across chemicals have been reviewed to determine the toxic nature of target chemical 4-(Diethylamino)azobenzene (2481 -94 -9). The studies are as mention below:
In the first weight of evidence study for 4-(Diethylamino)azobenzene (2481 -94 -9), Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna was predicted for 4-(Diethylamino)azobenzene (2481 -94 -9). The EC50 value was estimated to be 12.80 mg/l when 4-(Diethylamino) azo -benzene exposed to Daphnia magna for 48hrs.
Similarly in the second weight of evidence study for RA chemical (86 -30 -6), j-check. Acute Immobilization Test of N-Nitrosodiphenylamine to Daphnia magna. Test was performed in the static system in which different concentration 3.00, 5.40, 9.30, 18.0, 30.0 mg/L was taken. Nominal values were adopted for calculation of effect concentration, because all measurement concentrations of test Substance which were measured at the start of exposure and after 48 hours were within ±20 % of nominal values.). After 24 hrs, Median immobilization concentration (EiC50) was 13.5 mg/L (95 % CL: 9.30 – 18.0 mg/L), No observed effect concentration (NOECi): 9.30 mg/L. And Lowest concentration of 100 % inhibition was 30.0 mg/L. But after 48 hrs. Exposure no observed effect concentration (NOECi) was 3.00 mg/L. And the lowest concentration of 100 % inhibition: 18.0 mg/L. Based on the immobilization of daphnia magna, due to the N-Nitrosodiphenylamine, the EC50 was 10.1 mg/l
Similarly in the third weight of evidence study for the RA chemical (140 -56 -7) ecotox, Study of short term toxicity of N-[[4-(Dimethylamino) phenyl]imino] sulfamic acid sodium salt on the growth of invertebrates. The study was carried out in the static system for 24 hrs.Based on the mortality of Gammarus fasciatus due to the N-[[4-(Dimethylamino)phenyl]imino]sulfamic acid sodium salt dye to the 24 hrs exposure the LC50 was 18 mg/l. Based on the LC50, N-[[4-(Dimethylamino) phenyl]imino] sulfamic acid sodium saltchemical was toxic and can be consider to be not classified as per the CLP classification criteria.
Based on the various experimental data and prediction data for the target chemical and read across chemicals, 4-(Diethylamino)azobenzene (2481 -94 -9) was toxic and can be consider to be toxic and can be classified in aquatic chronic 3 category.
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