3-(2,4-bis(4-((5-(4,6-bis(2-aminopropylamino)-1,3,5-triazin-2-ylamino)-4-hydroxy-2,7-disulfonaphthalen-3-yl)azo)phenylamino)-1,3,5-triazin-6-ylamino)propyldiethylammonium lactate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June 17th to 21st, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

April 4th, 1984

GLP compliance:

yes (incl. QA statement)

Test organisms (species):

activated sludge of a predominantly domestic sewage

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test temperature:

21 °C

pH:

pH negative control = 8.0 - 8.2
pH test substance = 8.0 - 8.2
pH positive control = 8.0

Nominal and measured concentrations:

Nominal concentrations: 3.2, 10, 32, 50, 100 mg/l

Details on test conditions:

Continuous aeration during the 30 minutes test period using compressed air at an air-flow rate of approximately 0.2 l per minute.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol at 1, 3.2, 10, 32, 50 mg/l

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

The respiration rate of aerobic waste water bacteria was not inhibited (-7.1 % to -12.3 %) when exposed to the test article conoentrations in the range from 3.2 to 100 mg/l. Based on these results the EC50 (30 minutes) can be defined as >100 mg/l.

Results with reference substance (positive control):

The respiration rate of the activated sludge was not inhibited (-2.8 % and -1 .9 %) at the two lowest rconcentrations of 1.0 and 3.2 mg/l. At 10, 32 and 50 mg/l, the respiration rate was inhibited by 21.0, 67.6 and 73.7 %, respectively. The EC50 (30 minutes) determined by linear regression was calculated to be 20.5 mg/l. The results confirmed the suitability of the activated sludge and method used.

Validity criteria fulfilled:

yes

Conclusions:

EC50 (30 minutes) > 100 mg/l.

Executive summary:

The inhibitory effect of the test substance on aerobic waste-water bacteria was investigated in a respiration test according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test).
The compound showed an EC 50 value >100 mg/L.

Description of key information

EC50 (30 minutes) > 100 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

The inhibitory effect of the test substance on aerobic waste-water bacteria was investigated in a respiration test according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test).
The compound showed an EC 50 value >100 mg/L.