Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 424-310-4 | CAS number: 178452-66-9 BLUE PE 3811
Endpoint summary
Administrative data
Description of key information
Short-term acute toxicity to fish
LC50 (96 h) > 200 mg/L
Short-term acute toxicity to aquatic invertebrates
EC50 (48h) >200 mg/L
Toxicity to aquatic algae and cyanobacteria
EC50 (72 h) > 161 mg/L
Toxicity to microorganisms
EC 50 value >100 mg/L
Additional information
Short-term acute toxicity to fish
The short-term acute toxicity to fish species was carried out according to scientifically acceptable methods comparable to OECD test guideline 203 and EU Method C.1 (Acute Toxicity for Fish).
The LC50 estimation for a 96-hour exposure of the test substance is 200 mg/L.
Short-term acute toxicity to aquatic invertebrates
The short-term acute toxicity to aquatic invertebrates was determined according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).
NOEC (48 h) = 200 mg/L
LOEC (48 h) > 200 mg/L
EC50 (48h) >200 mg/L
Toxicity to aquatic algae and cyanobacteria
The influence of the test item on the growth of the green algal species Scenedesmus subspicatus was investigated in a 72-hour static test according to EU Method C.3 (Algal Inhibition test), and the OECD Guideline No.201, 1984. The 72-hour NOEC was determined at the test substance concentration of 65 mg/l (nominal 91 mg/l).
The test concentrations corresponding to 10, 50 and 90 % inhibition of the algal biomass (EC10, 50, 90) and of the growth rate could not be calculated due to the low growth inhibition effect up to the hightest concentration. The 72-hour EC 50-biomass and the 72-hour EC 50-growth rate were clearly higher than 161 mg/I.
Toxicity to microorganisms
The inhibitory effect of the test substance on aerobic waste-water bacteria was investigated in a respiration test according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test).
The compound showed an EC 50 value >100 mg/L.
Justification for classification or non-classification
According to the CLP Regulation (EC 1272/2008), Table 4.1.0 b) iii) a substance should be classified in Category 3 when L(E)C50 values for fish, daphnia and algae are between 10 and 100 mg/L. In the available aquatic toxicity studies, the EC50 values are always above 100 mg/L therefore, the substance is not classified for aquatic toxicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
