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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 9th to 11th, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
December 29th, 1992
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
Aprile 4th, 1984
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
The nominal concentrations tested were 9, 19, 41, 91 and 200 mg /l test substance. Additionally, a control group (test water without addition of the test substance) was tested in parallel. The analytically determined test substance concentrations in the analysed test medium of 200 mg/l varied in the range from 95 % to 105 % of the nominal value. In the test medium the test substance was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results are related to the nominal concentration of the test substance.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
20 daphnids tested per control
20 daphnids tested per each test concentration

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg CaCO3/l
Test temperature:
The test media temperatures ranged from 20.4 to 20.8 °C.
pH:
The measured pH-values in the test media ranged from pH 7.1 to pH 7.9.
Dissolved oxygen:
>= 8.3 mg/l
Nominal and measured concentrations:
Nominal concentrations of the test substance = 9, 19, 41, 91, 200 mg/l.
The analytically determined test substance concentrations in the analysed test medium of 200 mg/l varied in the range from 95 % to 105 % of the nominal value. In the test medium the test substance was sufficiently stable during the test period of 48 hours. Therefore, all reported biological results are related to the nominal concentration of the test substance.
Details on test conditions:
In Milli-Q-water salts were added: CaCl2 × 2H2O (2.0 mmo/L = 294.0 mg/l), MgSO4 × 7H2O (0.5 mmol/L = 123.0 mg/l), NaHCO3 (0.75 mmol/l = 65.0 mg/l), KCI (0.75 mmol/l = 5.8 mg/l). Water was aerated to oxygen saturation.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
In the control and up to and including the test concentration of 200 mg/l of the test substance no significant rate of immobility or mortality of the test animals was observed during the test period of 48 hours. The immobilization rate of 10 % in the control does not have any influence on the validity of the test because according to the test guidelines this immobilization rate is tolerated in the control and additionally, no other signs of intoxication were observed. In all the test concentrations the test animals were coloured by the test substance. However, this colouration of the Daphnids had no detrimental impact on the survival rate or mobility of the test animals. Therefore, the 48-hour NOEC and the 48-hour EC 0 of Blue PE 3811 to Daphnia magna were determined to be at least 200 mg/l of the test substance. The NOEC might even be higher than this concentration. The 48-hour LOEC, the 48-hour EC 50 and the 48-hour EC 100 were clearly higher than this test concentration. These values could not be quantified, since concentrations in excess of nominal 200 mg/l of the test substance have not been tested. All test media were coloured by the test substance. After 48 hour test duration a small part of the test substance had settled on the bottom ofthe glass beaker and an adsorption of the test substance onto the walls of the test beakers was observed.

Applicant's summary and conclusion

Conclusions:
The test substance has no toxic effects on the daphnids up to 200 mg/l concentration.