Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 29, 2013 - November 7, 2013
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was performed in accordance with the EC A.8 and OECD 117 guidelines and complied with the principles of GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Version / remarks:
(2008)
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
Version / remarks:
(2004)
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 830.7570 (Partition Coefficient, n-octanol / H2O, Estimation by Liquid Chromatography)
Version / remarks:
(1996)
Deviations:
no
GLP compliance:
yes
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): KF-1622
- Description: Clear colourless liquid
- Storage condition of test material: In refrigerator (2-8°C) in the dark
- Expiration date of the lot/batch: May 17, 2014

Results and discussion

Partition coefficientopen allclose all
Type:
log Pow
Partition coefficient:
> 7.2
Temp.:
35 °C
Remarks on result:
other: Non-buffered mobile phase
Type:
Pow
Partition coefficient:
> 16 000 000
Temp.:
35 °C
Details on results:
- In the chromatogram of the test solution, one major peak and 3 smaller test substance peaks were observed.
- For the major peak (Peak area 98.5%) a retention time of 29.19 minutes was observed.
- Under the same analytical conditions, the retention time of Benzo[ghi]perylene (log Pow = 7.2) was 18.23 minutes.

Any other information on results incl. tables

Results HPLC method

Substance

tr,1
[min]

tr,2
[min]

mean tr
(n=2)

log Pow

Pow

Area
%

4,4’-DDT

11.402

11.464

11.433

6.5

 

 

Benzo[ghi]perylene

18.203

18.249

18.226

7.2

 

 

 

 

 

 

 

 

 

Test substance_peak 1

29.084

29.074

29.079

> 7.2

1.6 x 107

0.2

Test substance_peak 2

29.191

29.182

29.187

> 7.2

1.6 x 107

98.5

Test substance_peak 3

29.388

29.376

29.382

> 7.2

1.6 x 107

1.0

Test substance_peak 4

29.584

29.569

29.577

> 7.2

1.6 x 107

0.3

Remarks:

- Log Pow value for Benzo[ghi]perylene according to Verschueren, K. (1996) Handbook of Environmental Data on Organic Chemicals, 3rd ed. Ultra Scientific, North Kingstown, USA

- Log Pow value for 4,4’-DDT according to OECD 117

Applicant's summary and conclusion

Conclusions:
The log Pow of the substance has been determined as >7.2 using the HPLC method (GLP-compliant study in accordance with EC A.8, OECD 117 and EPA OPPTS 830.7570). The HPLC chromatogram of the test substance showed one major (98.5% area) and 3 smaller peaks; all with a retention time > reference substance Benzo[ghi]perylene (log Pow of 7.2). It was therefore concluded that the log Pow of the substance is >7.2.