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EC number: 608-670-1 | CAS number: 31831-53-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 July 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Confirmatory analysis is inadequate for test solutions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- A sample of 1 ml was taken from the old and new test solutions of the highest four concentrations on each day of the study. These samples were qualitatively analyzed by HPLC. As Capa-203 consists of a mixture of components the chromatogram measured at 218 nm shows a number of peaks.
As no samples of the pure components (i.e. analytical standards) of Capa-203 were available no quantitative conclusions could be drawn.
The chromatogram of freshly prepared and 24 hours old solutions were only compared qualitatively.
From each freshly prepared clear saturated solution (stock solution) a sample of 50 ml was taken for determination of the total organic carbon content (TOC). These determinations were done by Centrilab, Soest, The Netherlands. To avoid precipitation the solutions were diluted 1:1 with ISO-water before shipment. - Vehicle:
- no
- Details on test solutions:
- The test was conducted using semi-static conditions (media renewal every 24 hours).
In order to simulate an accidental spill a saturated solution of Capa-203 was made by adding 6.5 g Capa-203 to 6.5 L of ISO-water under stirring by an ultra-turrax to provide a nominal stock solution at 1 g/L. Stirring was continued for 10 minutes which resulted in a turbid emulsion.
A clear solution was obtained by filtration through a nylon membrane filter (Gelman, Nylaflo, 0.45 pm) under vacuum. Dilutions were made by making up 1500, 750, 375,187 and 95 mL of the saturated solution to 3 L with ISO-water.
A control with ISO-water was included in the test. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The zebra fish used on the study originated from a commercial supplier, Ruinemans, Montfoort, The Netherlands. The batch of fish were received on the 20 May 1992 and held in a plastic container 40 x 60 x40 cm containing ca. 80L of reconstituted ISO-water which was continuously filtered and aerated. The aquaria were kept in a room at 21 ± 1˚C.
The fish were fed trout feed and Daphnia (waterfleas).
Stock fish held at 21 ± 1˚C test conducted at ca. 23˚C.
The fish used in the final test were 2.3 - 2.9 cm long and had a mean weight of 0.25 g per fish. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- Hardness of ISO reconstituted water ca. 250 mg/L.
- Test temperature:
- The temperature of the test solutions varied between 22.0 and 23.8 °C.
- pH:
- The pH of the test solutions ranged from 7.3 to 8.0 during the test, except in one aquarium with dead fishes where the pH was 6.6.
- Dissolved oxygen:
- The dissolved oxygen concentration was between 5.3 and 9.5 mg/L.
In the aquarium with dead fishes (140 mg/L) the O2 concentration dropped to 1.8 mg/L. - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0, 19, 38 , 75, 150, 300 and 600 mg/L.
Measured concentrations: 0, 18, 35, 70, 140, 289 and 560 mg/L (Based on TOC analysis of initial saturated solution and calculation).
As % nominal: NA, 95, 92, 93, 93, 96 and 93%. - Details on test conditions:
- The initial saturated stock was prepared to simulate and accidental spill.
Test media reconstituted ISO water:
Deionised water
CaCl2.2H2O - 294 mg/L
MgSO4. 7H2O - 123 mg/L
NaHCO3 - 64 mg/L
KCl - 5.8 mg/L
pH 7.8
Test solutions were aerated during the test and the light regime was 16 h light and 8 h dark. - Reference substance (positive control):
- yes
- Remarks:
- Potassium bichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 72 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Total organic carbon
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 35 and 140 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Total organic carbon
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Total organic carbon
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 18 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Total organic carbon
- Basis for effect:
- behaviour
- Details on results:
- All fishes exposed to 280 and 560 mg Capa-203/1 were dead within 3 hours, and were removed from the aquaria. All fishes exposed to 140 mg/L died within 24 hours, but stayed in the aquarium during this whole period which influenced the pH and O2 concentration of the test solution.
40% mortality occurred after 96 hours when zebra fishes were exposed to 70 mg Capa-203/L. At lower concentrations no mortality was observed.
As the mortality in the controls is below 10%, the study meets the criterion.
Sub-lethal observations were identified at all test concentrations during the test, the sub lethal effects were uncontrolled and hypoactivity. - Results with reference substance (positive control):
- Once a year an acute toxicity test with Brachydanio rerio and the reference substance, potassium bichromate, is conducted. The most recent test (as of the date when the current study was performed) was conducted in April 1992. The 96 h EC50 found in this reference test was 142 mg/L (study number C.REF.51.006b).
- Reported statistics and error estimates:
- The percentage mortality was plotted against the test concentration using a probit analysis model. From the results it was concluded that the 96-h LC50 was 72 mg/L, with confidence limits of 35 and 140 mg/L.
- Sublethal observations / clinical signs:
Fish mortality and sub-lethal observation during the study are presented below.
Calculated test concentrations (mg/L)
Number of surviving zebra fish per test vessel
% mortality
0 h
3 h
24 h
48 h
72 h
96 h
0 (control)
10
10
10
10
10
10
0
18
10
10
10
104)
104)
105)
0
35
10
10
10
103)
103)
106)
0
70
10
101)
7
63)
63)
63)
40
140
10
12)
0
0
0
0
100
280
10
0
0
0
0
0
100
560
10
0
0
0
0
0
100
1) one fish showed uncontrolled movement
2) one fish showed hypo activity
3) all fishes showed uncontrolled movement and hypo activity
4) 5 fishes showed uncontrolled movement and hypo activity
5) 2 fishes showed uncontrolled movement and hypo activity
6) 4 fishes showed uncontrolled movement and 10 fishes showed hypo activity
- Validity criteria fulfilled:
- yes
- Conclusions:
- Zebra fish were exposed to Capa-203 at calculated concentrations of 18, 35, 70, 140, 280 and 560 mg/L in a semi-static 96 -hour acute toxicity test according to OECD 203 guidelines. The test design included an untreated control comprising of ISO dilution water only.
The 96-hour LC50 determined was 72 mg Capa-203/L (based calculated concentrations). - Executive summary:
Zebra fish were exposed to Capa-203 at calculated concentrations of 18, 35, 70, 140, 280 and 560 mg/L in a semi-static 96-hour acute toxicity test according to OECD 203 guidelines. The test design included an untreated control comprising of ISO dilution water only.
The 96-hour LC50 determined was 72 mg Capa-203/L (based calculated concentrations).
This test was conducted in accordance with the 1984 version of the OECD 203 guideline. The current guideline of 1994 states that there must be evidence that the concentration of the substance being tested has been satisfactorily maintained and preferably it should be at least 80% of the nominal concentration throughout the test. This reported study was conducted prior to the issue of the 1994 OECD 203 document and therefore samples of test media have not been determines using an appropriate analytical method.
Reference
Description of key information
The key study for acute toxicity of 2-Oxepanone, polymer with 1,4-butanediol to fish found a 96-hour LC50 of 72 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 72 mg/L
Additional information
Zebra fish were exposed to Capa-203 at calculated concentrations of 18, 35, 70, 140, 280 and 560 mg/L in a semi-static 96-hour acute toxicity test according to the OECD 203 guideline. The test design included an untreated control comprising of ISO dilution water only.
The 96-hour LC50 determined was 72 mg Capa-203/L (based on calculated concentrations).
This test was conducted in accordance with the 1984 version of the OECD 203 guideline. The current guideline of 1994 states that there must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the test. This reported study was conducted prior to the issue of the 1994 OECD 203 document and therefore samples of test media have not been determines using an appropriate analytical method.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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