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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
December, 2012 to April, 2013
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study was conducted by a GLP accredited laboratory using OECD Testing Guideline 203.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Cocoa Powder (Low Fat)
IUPAC Name:
Cocoa Powder (Low Fat)
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Test substance name: Cocoa Powder (10 - 12% lipid)
Batch number: ALF092012
Description: Brown solid
Purity: Not supplied
Storage: Room temperature (15 - 30°C), desiccated and protected from light

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Healthy Oncorhynchus mykiss (rainbow trout) were supplied by Brow Well Fisheries, United Kingdom, details of which are maintained in Smithers Viscient (ESG) Ltd. records. The fish were acclimatised to the test conditions for at least 12 days prior to testing. The mortality rate of the stock batch of fish was 0% in the seven days prior to the definitive test. Fish were selected at random from the holding tank and allocated to the test vessels at the start of each phase of testing. The fish were not fed for at least 24 hours before the start of the definitive test and were not fed throughout the test. At the end of the test, the total length and wet weight of ten fish, selected at random from those used in the test, were determined.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
72 mg/l at the start of the test.
Test temperature:
14.5ºC at the start of the test.
pH:
7.11 at the start of the test.
Dissolved oxygen:
9.04 mg/l at the start of the test.
Nominal and measured concentrations:
The test was conducted at a single nominal test substance concentration of 100 mg/l.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOELR
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Parameter

Toxicity Value (mg/l)

24 hours

48 hours

72 hours

96 hours

LL50

>100

>100

>100

>100

NOELR

100

100

100

100

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
The validity criteria for control mortality and dissolved oxygen (>60% ASV) were both satisfied. The test was therefore considered to be valid.
Conclusions:
Based on nominal concentrations, the 96-hour NOELR was considered to be >100 mg/l loading rate Watre Accomodated Fraction (WAF). It is concluded that Cocoa Powder (10 - 12% lipid) was considered not to be toxic to Oncorhynchus mykiss at 100 mg/l loading rate WAF under the conditions of this test.
Executive summary:

The potential for the substance to be acutely toxic to fish was determined in accordance with the OECD Guideline for Testing of Chemicals 203. The study was conducted using a semi-static test design with daily renewal of test media during the test. Due to the complex nature of the test substance, it was considered that a Water Accommodated Fraction (WAF) approach was the most suitable method of test media preparation. Based on the results of the range-finding test, which is not fully reported, a definitive test was conducted at a single nominal test substance concentration of 100 mg/l loading rate WAF. Based on nominal concentrations, the 96-hour NOELR was considered to be >100 mg/l loading rate WAF. It is concluded that Cocoa Powder (10 - 12% lipid) was considered not to be toxic to Oncorhynchus mykiss at 100 mg/l loading rate WAF under the conditions of this test.

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