Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: 97.0 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test item was mixed with water at 30°C and homogenised using an Ultra-Turrax and by ultrasonic treatment.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Strain: Hamilton-Buchanan
- Source: West Aquarium Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): 3 months
- Length at study initiation (mean, range and SD): 2.8 cm, 2.4 - 3.4 cm, 0.20 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Type and amount of food:
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h

Test conditions

Test temperature:
Test groups: 21.0 - 22.8 °C
Control groups: 21.6 - 22.7 °C
pH:
Test groups: 6.4 - 8.0
Control groups: 7.3 - 8.1
Dissolved oxygen:
Test groups: 6.2 - 8.8 mg/l
Control groups: 7.0 - 9.3 mg/l
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
350 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
>= 500 - <= 710 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 500 - <= 710 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
710 mg/L
Nominal / measured:
nominal
Details on results:
A probit analysis to determine the LC50 was not possible given the distribution of the effective concentrations and the dilution factors used.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
From this study, LC0 (48 h and 96 h) for 2-chlorobenzotrichloride was determined to be 350 mg/L, LC50 (48 h and 96 h) to be 500 - 710 mg/L and LC100 (496 h) to be 710 mg/L.