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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23-06-1981 to 24-06-1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38 412 Part 11
Version / remarks:
related to OECD Guidelline 202, Guideline 84/449/EWG (C2) and ISO 6341 but the incubaiton time is different (24 hrs vs 48hrs)
Deviations:
not specified
GLP compliance:
no
Remarks:
GLP not in force at the time of the study

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Specific details on test material used for the study:
A saturated solution preapred under hydrolytic conditions for the test-substance: A suspension of the substance in de-ionised water was adjusted to pH 12 with NaOH, stirred for 72 hours at 25C and filtered.
Analysis of stock solution: 304 mg DOC/L
concnertation of stock solution

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Details on test solutions:
Analysis of stock solution: 304 mg DOC/L
concentration of stock solution 831.4 mg/L calculated fro DOC of the solution and carbon contnet ofthe substance of 36.57%

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: straus
- Source: own laboratory strain
- Age of parental stock (mean and range, SD): not given
- Feeding during test -not given
- Food type: algae (Scenedesmus supspicatus)
- Amount:
- Frequency: twice daily

Breeding: cultivated adult animals in glass vessels (4L) canging the medium once a week and feeding the animals twice a day. Medium : Synthetic water according to DIN 38 412 part 11


Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Hardness:
as per DIN 38 412 part 11
Test temperature:
21 C thermostatically controlled
pH:
as per DIN 38 412 part 11
Dissolved oxygen:
not determined
Salinity:
as per DIN 38 412 part 11
Conductivity:
as per DIN 38 412 part 11
Nominal and measured concentrations:
Nominal concentrations: 8.31, 83.1, 103.9, 166.3, 415.7, 831.4 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100ml glass vessel
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 100mL
- Aeration: not specified
- Type of flow-through (e.g. peristaltic or proportional diluter): N/A
- Renewal rate of test solution (frequency/flow rate): N/A
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 10 animals per vessel

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water according to DIN 38 412 part 11
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: conducted in dark
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : motility

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes
Remarks:
potassium dichromate 2mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
ca. 112 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
ca. 210 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC90
Effect conc.:
ca. 394 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
70% mean immobilised daphnia following 24hr exposure to 2mg/L potassium dichromate
Reported statistics and error estimates:
EC values calculated by logarithmic regression of the measured data
EC10 95% CI = 42-131 mg/L standard error 104-118 mg/L
EC50 95% CI = 188-417 mg/L standard error = 202-221 mg/L
EC90 95% CI= 271-4203 mg/L standard error = 346-472 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Considering the incubation time of only 24 hours it cannot be excluded that a test according to OECD202 would result in a range of 10-100 mg/L for the EC50 value of the hydrolysed solution of the test substance to Daphnia magna