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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
7.2. 2000 - 30.3.2000
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: B.14 Mutagenicity, test of reversal mutagenicity, Salmonella typhimurium OECD NO. 471
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Test batch: No.. 7/99

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonella typhimurium : (TA1537, TA98, TA100 , TA1535)
Metabolic activation:
with and without
Metabolic activation system:
The simulation of the absorption metabolism was carried out by S9 fraction of the liver homogenate and the co-factors mixture.
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): .01 ... .5 µg/plate
Concentration range in the main test (without metabolic activation): .01 ... .5 µg/plate
Vehicle / solvent:
Solvent: dimethylsulphoxid

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Untreated negative controls validity:
valid
Positive controls validity:
valid
Additional information on results:
Observations:
According to the mentioned results Dusantox L is not

mutagenic, by test with metabolic as without metabolic
activation.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

The test results : whithout the bacterial backround changes - all concentration are not toxic.