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Short-term toxicity to aquatic invertebrates

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Description of key information

Daphnia Magna, 48 -hours, test medium not adjusted, EC50 = 3.0 mg/L (95% confidence interval between 2.8 and 3.2 µg/L) (values based on measured initial di-ester concentrations).


This available 48h-EC50 value for the substance based on the di-ester (3 mg/L) represents a Water Soluble Fraction prepared at a loading rate between 18 and 32 mg/L.

Key value for chemical safety assessment

Additional information

The short-term toxicity of the substance to aquatic invertebrates was determined in a daphnia acute study according to OECD guiudeline No. 202. In addition, procedures were based on the test method described in the OECD series on testing and assessment number 23, 2019.


A final test was performed based on the results of a preceding combined limit/range-finding test. Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an untreated control and to WSFs individually prepared at loading rates of 10, 18, 32, 56 and 100 mg/L. The total exposure period was 48 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test.


Samples taken from all test concentrations and the control were analysed by following the mono-ester (m/z 209.1) and di-ester (m/z 321.1). The measured concentrations of the mono‑ester were at the level of nominal throughout the exposure period, i.e. were at 97 – 105% of nominal at the start and end of exposure. The measured concentrations of the di-ester were 0.94, 2.4, 3.8, 5.4 and 7.7 mg/L at the start of the test, respectively to the nominal loading rates of 10, 18, 32, 56 and 100 mg/L. The concentrations remained stable, i.e. were at 95 – 103% relative to initial at the end of the test. Based on these results, effect parameters were expressed as initially measured concentrations of the di-ester, since the lower measured concentrations represent the worst case scenario.


At the end of the test, a dose-related increase of immobility was observed at WSFs individually prepared at a loading rate of 18 mg/L and higher, reaching≥95% immobility at the three highest test concentrations.


In conclusion, the 48h, EC50 for Daphnia magna exposed to the substance was 3.0 mg/L based om measured concentrations (95% confidence interval between 2.8 an 3.2 mg/L). This 48h-EC50 value for the substance based on the di-ester (3 mg/L) represents a Water Soluble Fraction prepared at a loading rate between 18 and 32 mg/L.


The study met the acceptability criteria prescribed by the study plan and was considered valid.