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EC number: 949-859-1 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Fresh water algae study, 72 Hours, ERC10 = 0.24 mg/L, ERC50 = 1.2 mg/L, NOEC = 0.13 mg/L.
This 72h-ErC50 value for the substance based on the di-ester (1.2 mg/L) represents a Water Soluble Fraction prepared at a loading rate of 10 mg/L and this 72h-ErC10 value for the substance based on the di-ester (0.24 mg/L) represents a Water Soluble Fraction prepared at a loading rate between 1 and 3.2 mg/L.
Key value for chemical safety assessment
Additional information
A 72 hour toxicity study with freshwater algae (Pseudokirchneriella subcapitata ) was conducted according to OECD guideline 201 and GLP principles.
The algae were exposed toWSFs individually prepared at loading rates of 1.0, 3.2, 10 and 32 mg/L,and solutions containing 10 and 32% of the WSF prepared at a loading rate of 1.0 mg/L.
Samples taken from all test concentrations and the control were analysed by following the mono-ester (m/z 209.1) and di-ester (m/z 321.1).The measured concentrations of the mono-ester were 0.092, 0.32, 0.98, 3.3, 9.9 and 32 mg/L at the start of the test, respectively to the 10 and 32% dilutions of the 1.0 mg/L WSF and the loading rates of 1.0, 3.2, 10 and 32 mg/L. The concentrations remained generally stable, i.e. the 1.0 to 32 mg/L WSFs were at 82-94% relative to initial at the end of the test, however the 10 and 32% dilutions of the 1.0 mg/L WSF decreased to 72-73% of initial at the end of the test.
The measured concentrations of the di-ester were 0.010, 0.039, 0.13, 0.40, 1.2 and 3.4 mg/L at the start of the test, respectively to the 10 and 32% dilutions of the 1.0 mg/L WSF and the loading rates of 1.0, 3.2, 10 and 32 mg/L. The concentrations remained stable, i.e. were at 100-111% relative to initial at the end of the test.
Based on these results, effect parameters were expressed as initially measured concentrations of the di-ester, since the lower measured concentrations represent the worst case scenario.
The substance inhibited growth rate of this freshwater algae species significantly at measured concentrations of 0.40 mg/L and higher.The 72h-EC50 for growth rate inhibition (ERC50) was 1.2 mg/L with a 95% confidence interval ranging from 1.1 to 1.4 mg/L. The 72h-NOEC for growth rate inhibition was 0.13 mg/L. The 72h-EC10 for growth rate inhibition was 0.24 mg/L.
This 72h-ErC50 value for the substance based on the di-ester (1.2 mg/L) represents a Water Soluble Fraction prepared at a loading rate of 10 mg/L and this 72h-ErC10 value for the substance based on the di-ester (0.24 mg/L) represents a Water Soluble Fraction prepared at a loading rate between 1 and 3.2 mg/L.
The study met the acceptability criteria, and is considered to be valid.
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