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EC number: 815-706-1 | CAS number: 20767-00-4
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 1.0 to 100 mg/L (nominal). Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
32 – 100 mg/L (nominal)
At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC.
The measured concentrations lay between 95 % and 104 % of the nominal concentrations at the beginning of the test and between 97 % and 103 % of the nominal concentrations at the end of the test. Therefore, the biological results were based on the nominal concentrations (see Guidance Doc. No. 23 §176).
Due to the low inhibition levels no EC50 could be determined for growth rate. Therefore, this value is given as a range.
The 72h-EC50values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.65 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
One study was performed according to OECD 201.
Based on the test results the NOEC, 72 h can be stated to be 10.00 mg/L and the EC50, 72 h can be stated to be > 100 mg/L, respectively.
Due to the results of the study, the test material does not meet the criteria for a classification as acute or chronic aquatic toxic.
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