Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The keeping is performed similar to the method described in the OECD guideline, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“), version 12 from 02. Feb. 2015.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20.5 - 21.2 °C
pH:
6.3 - 7.8
Dissolved oxygen:
8.6 - 8.8 mg/L
Nominal and measured concentrations:
Nominal concentration: 100 mg/L
The concentration determined at the start of the test was 104 % of the nominal concentration. At the end of the test the determined concentration was 115 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration. The increase in concentration at the end of the test may be due to evaporation and thus concentration of the test solution.
Details on test conditions:
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Conclusions:
Based on the test results the NOEC, 48 h can be stated to be >= 100 mg/L and the EC50, 48 h can be stated to be > 100 mg/L, respectively.
Due to the results of the study, the test material does not meet the criteria for a classification as acute or chronic aquatic toxic.
Executive summary:

One valid experiment was performed.

The study was performed as a limit test at 100 mg/L nominal concentration. For each test concentration and the blank control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

None of the animals was immobilised in the blank control or the treatment.

Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.

 

At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-determination. The concentration determined at the start of the test was 104 % of the nominal concentration. At the end of the test the determined concentration was 115 % of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration. The increase in concentration at the end of the test may be due to evaporation and thus concentration of the test solution.

No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.

The result of the test is considered valid.

Description of key information

One study was performed according to OECD 202.

Based on the test results the NOEC, 48 h can be stated to be >= 100 mg/L and the EC50, 48 h can be stated to be > 100 mg/L, respectively.

Due to the results of the study, the test material does not meet the criteria for a classification as acute or chronic aquatic toxic.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information