(3E)-1,7,7-trimethyl-3-(4-methylbenzylidene)bicyclo[2.2.1]heptan-2-one

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31 March 1998 to 04 April 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Concentration measurments only at t=0

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Deviations:

not specified

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0.1 g/L
- Sampling method: The test material concentration was analysed at the beginning of the study

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test media (reconstituted water and test material) were freshly prepared. The calibrated flask was treated in an ultrasonic device for 1 hour. Subsequently the preparation was aerated and stirred with a magnetic stirrer for 23 hours. After 24 hours the solution was filtered with a nutsch filter (pore size 10 to 16 µm).
- Controls: Reconstituted water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: CRL/ZF 1
- Age at study initiation: Approximately 29 weeks

ACCLIMATION
- Acclimation period: 7 days
- Type and amount of food during acclimation: Tetra Min diet
- Feeding frequency during acclimation: Fish were fed during the acclimation, until one day before the start of the experiment.
- Health during acclimation: Less than 5% mortality in the test fish batch.

FEEDING DURING TEST: None

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Hardness:

250 mg/L as CaCO3

Test temperature:

23°C

pH:

7.8 to 8.1

Dissolved oxygen:

92.2 to 97.7% oxygen saturation

Salinity:

n/a

Conductivity:

Not reported

Nominal and measured concentrations:

Nominal: 0.1 g/L
Measured (start of the test): 0.00074 g/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquarium
- Material, size, headspace, fill volume: 6 L test solution
- Aeration: No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 600 mL/fish

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fully demineralised water
- Culture medium different from test medium: No
- Intervals of water quality measurement: At the beginning of the exposure period and then daily.

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light, 12 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The behaviour and general condition of all fish were checked immediately after the introduction into the test vessel, 3 and 6 hours later, and then daily.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 0.74 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 0.74 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 0.74 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.74 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: Signs of intoxication (slightly irregular swimming, near the water surface, near the bottom of the aquarium, lying on the back, gasping) were seen 3 hours after exposure up to the end of the study.
- Mortality of control: None
- Other adverse effects control: None

Sublethal observations / clinical signs:

Table 1. Mortality results

Concentration (mg/L)	Number of dead fish
	Immediately after test start	3 h	6 h	24 h	48 h	72 h	96 h
Control	0	0	0	0	0	0	0
0.74	0	0	0	3	3	3	3

Validity criteria fulfilled:

not specified

Conclusions:

The 96-hour LC50 was > 0.74 mg/L for Brachydanio rerio.

Executive summary:

The acute toxicity of the test item to zebrafish (Brachydanio rerio) was determined in a 96 -hour static limit test conducted according to OECD Guideline 203 and EU Method C.2. Fish were exposed to a nominal concentration of 100 mg/L of the test item (measured concentration 0.74 mg/L) in reconstituted water, alongside a control group. The test solution was prepared by ultrasonication of the test item in the test media for 1 hour, followed by aeration and stirring for 23 hours. The solution was filtered before use. Each treatment contained one replicate with 10 fish. The 96 -h LC50 was determined to be > 0.74 mg/L. This test is reliable with restriction (Klimisch 2) as test concentrations were only measured at t = 0h.

Description of key information

Based on measured concentrations, the 96-hour LC50 was > 0.74 mg/L for Brachydanio rerio.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.74 mg/L

Additional information

The acute toxicity of the test item to zebrafish (Brachydanio rerio) was determined in a 96 -hour static limit test conducted according to OECD Guideline 203 and EU Method C.2 (1998). Fish were exposed to a nominal concentration of 100 mg/L of the test item (measured concentration 0.74 mg/L) in reconstituted water, alongside a control group. The test solution was prepared by ultrasonication of the test item in the test media for 1 hour, followed by aeration and stirring for 23 hours. The solution was filtered before use. Each treatment contained one replicate with 10 fish. The 96 -h LC50 was determined to be > 0.74 mg/L.