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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Limitations in the reporting of experimental details and results
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
GLP compliance:
not specified
Remarks:
Published study from a peer-reviewed journal.
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Nominal and measured concentrations:
Five experimental concentrations between 0.1 and 1000 μg/L.
Details on test conditions:
After 48 h, motile and immobilized Daphnia magna were counted.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
The 48-hour EC50 for Daphnia magna was 0.56 mg/L.
Executive summary:

The test item was assessed in a 48-hour acute study with Daphnia magna. The study reports that OECD guideline 202 was followed, however limited methodological details are reported. Five test concentrations were used, between 0.1 and 1000 μg/L. The 48-hour EC50 for Daphnia magna was 0.56 mg/L. The study is assigned a Klimisch score of 2 (reliable with restrictions) as although it followed a standard test guideline the paper reports limited details on experimental design and results.

Description of key information

Based on nominal concentrations, the 48h EC50 for Daphnia magna was 0.56 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.56 mg/L

Additional information

The effects of the test item were determined in an acute immobilisation test with Daphnia magna, according to OECD 202 guideline (2011). The study was conducted under static conditions over a period of 48 hours with five nominal concentrations of the test item (0.4, 0.8, 1.6, 3.2, 6.4 mg/L). Twenty daphnids were exposed per treatment group (four replicates, each containing five daphnids). There was no analytical verification of the test item concentrations (although chemical analysis was undertaken for a chronic daphnid study conducted and reported in the same publication). Based on nominal concentrations, the 48h EC50 for Daphnia magna was 0.8 mg/L (95% confidence intervals: 0.72 - 0.88 mg/L).

In a second study (key study), the test item was assessed in a 48-hour acute study with Daphnia magna (2010). The study reports that OECD guideline 202 was followed, however limited methodological details are reported. Five test concentrations were used, between 0.1 and 1000 μg/L. The 48-hour EC50 for Daphnia magna was 0.56 mg/L. The study is assigned a Klimisch score of 2 (reliable with restrictions) as although it followed a standard test guideline the paper reports limited details.

As the results from both studies are similar, the study with the more sensitive result (EC50 = 0.56 mg/L) is taken as the key value for this endpoint.