Hexadecyltrimethylammonium toluene-p-sulphonate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Collection of bacetrial mutagenicity test results

Data source

Reference

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

Principles of method if other than guideline:

Six test substance concentrations; with and without metabolic activation; positive and negative controls; 3 replicates; application using preincubation assay with a 20 minute timeframe.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulphonic acid

EC Number:

202-638-7

EC Name:

Benzenesulphonic acid

Cas Number:

98-11-3

Molecular formula:

C6H6O3S

IUPAC Name:

benzenesulfonic acid

Method

Target gene:

Histidine independent mutant colonies of Salmonella typhimurium

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA97, TA98, TA100, TA1535

Additional strain / cell type characteristics:

other: histidine deficient

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254-induced S9 from rat and hanster

Test concentrations with justification for top dose:

100, 333, 1000, 3333, 6667 (without activation) and 10000 (with activation) micrograms per plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

no

True negative controls:

yes

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene; 4-nitro-o-phenylenediamine; sodium azide, 9-aminoacridine

Remarks:

DMSO as true negative control

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium, other: TA97, TA98, TA100, TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity, but tested up to precipitating concentrations

Vehicle controls validity:

not applicable

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Any other information on results incl. tables

Precipitation concentration 10,000 micrograms per liter

Applicant's summary and conclusion

Conclusions:

Benzenesulphonic acid is not cytotoxic or mutagenic in the Ames bacterial reverse mutation assay