Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 March, 1988 to 13 March, 1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other:
Remarks:
Study was conducted according to the OECD guideline 202 and in compliance with GLP.
Justification for type of information:
Direct read-across from C16 TMAC to Cetrimonium chloride is claimed as valid because they are both trimmoniums sharing a common alkyl chain length n=16. C16 TMAC is also known as the cetrimonium ion, exhibts properties and effects consistent with a category of quaternary ammonium compounds known as the alkyl trimoniums. C16 TMAC is commonly known as the cetrimonium ion. In pure form QACs also contain an anion such as chloride, bromide or methosulfate. When in solution the substances will be dissociated and the anion the QACs were once associated with will be irrelevant.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Light yellow liquid-Date received: 22 Feb, 1988
Analytical monitoring:
no
Details on sampling:
A clear stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask. The test solutions were prepared by adding the appropriate volume of stock solution to dilution water to total 1,000 mL. Each solution was mixed on a magnetic stirrer for 30 seconds and then divided into 4 beakers to provide replicate exposure treatments, each containing 200 mL. The remaining 200 mL of test solution were used for 0 h water quality measurement.
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION - Name of vehicle: Water - Concentration of vehicle in test medium (stock solution): Stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask
Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna- Source: Springborn Life sciences, Inc., Wareham, Massachusetts- Age at study initiation (mean and range, SD): ≤24 h- Feeding during test: NoACCLIMATION- Acclimation period: Cultures of the test organism over many generation has been continuously maintained at the lab- Acclimation conditions (same as test or not): Yes- Type and amount of food: Solution of green algae and yeast suspension daily- Feeding frequency: Daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
180 mg/L as CaCO3
Test temperature:
20-21⁰C
pH:
7.9-8.3
Dissolved oxygen:

98-99%
Nominal and measured concentrations:
236, 394, 667, 1,091, 1,818 and 3,030 µg/L as nominal concentration
Details on test conditions:
The test was conducted in 250 mL glass beaker. A clear stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask. The test solution was prepared by adding the appropiate volume of stock solution to dilution water to a total volume of 1 L. Each solution was mixed on a magnectic stirrer for 30 seconds and then divided into 4 beakers to provide replicate exposure treatments, each containing 200 mL. The remaining 200 mL of test solution was used for 0 hour water quality measurement and then discarded. One set of 4 control beakers was prepared containing the same dilution water and maintained under the same conditions as the exposure concentration, but without the test subsatnce. The ambient air temperature was controlled in order to maintain test solution temperature at 20 ± 1⁰C. The test solution was not aerated. The test area was illuminated with Sylvania GRO-LUX and cool white fluorescent lights at an intensity of 70 footcandles at the solution surface.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
550 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
280 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
< 236 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality, behavioural and physical abnormalities
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 0.09 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
< 0.08 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: mortality, behavioural and physical abnormalities
Details on results:
The 48 h EC50 and 95% confidence interval (calculated by probit analysis) was 280 (250-300) µg/L. The NOEC was <236 µg/L, the lowest tested concentration.
Validity criteria fulfilled:
yes
Conclusions:
The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 hour EC50and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L) , respectively (Surprenant DC, 1988).
Executive summary:

An OECD 202 study was conducted to evaluate the acute toxicity of the read-across substance C12-18 TMAC (active ingredient 33%) to Daphnia magna under static conditions. Daphnia magna (10 per dose) were exposed to the test substance at nominal concentrations of 236, 394, 667, 1,091, 1,818 and 3,030 µg/L for 48 hours and mortalities were determined. The 48 hour EC50 and 95% confidence interval were calculated by probit analysis as 280 (250 - 300) µg/L. No analytical determination of the test substance was performed. The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 hour EC50and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.

Description of key information

The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 hour EC50 and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L) , respectively (Surprenant DC, 1988).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
280 µg/L

Additional information