Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Study meets the criteria for Klimisch score 1 because it followed an internationally recognised method (OECD Guideline 301D) and was carried out to Good Laboratory Practice. However as this study is used in the context of a read across, Klimisch 2 is assigned.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: Benzenesulfonic acid C16-C24 alkaryl derivative, calcium salt
3. CATEGORY APPROACH JUSTIFICATION
Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge bacteria from domestic sewage treatment plant used at about 1 drop sludge filterate inoculum basal medium.

Duration of test (contact time):

28 d

Initial conc.:

2 mg/L

Based on:

COD

Parameter followed for biodegradation estimation:

other: COD

Details on study design:

Duplicate test vessels were used for the test substance, positive control and control blank. Test vessels were incubated at 20 ± 1°C.

Reference substance:

benzoic acid, sodium salt

Reference substance:

aniline

Test performance:

The positive controls were degraded by >60% within 28 days.

Key result

Parameter:

other: COD

Value:

8

Sampling time:

28 d

Remarks on result:

other: Additional sampling conducted on days 1, 3, 6, 10, 14 and 21 days.

Results with reference substance:

Sodium benzoate 97% after 28 days; aniline 61% after 28 days.

Validity criteria fulfilled:

yes

Remarks:

Both the reference substances achieved greater or equal to 60% degradation, and therefore, the test was valid.

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Only 8% biodegredation was observed after 28 days, therefore this substance is not Readily Biodegradable under testing conditions.

Executive summary:

A 28-day aerobic ready biodegradability study (to OECD Guideline No. 301D – Closed Bottle Test) was carried out using an analog of the submission substance (CAS Number 70024-71-4). The effects of the test substance on ready biodegradability were assessed based on the measurement of chemical oxygen demand (COD) using an activated sludge inoculum (from a domestic wastewater plant) that was not pre-adapted to the test substance. Test performance was evaluated using the reference substances sodium benzoate and aniline.

 

This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests.

 

Results Synopsis

 

There was only 8% degradation of the test substance after 28 days.

There was 97% and 61% degradation of the reference substances sodium benzoate and aniline after 28 days meaning the test was considered valid.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Study meets the requirements for Klimisch code 1 because it followed an internationally recognised method (OECD Guideline 301F) and was conducted according to Good Laboratory Practice. However as this study is used in the context of a read across, Klimisch 2 is assigned.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: Sulfonic acid, petroleum, calcium salt
3. CATEGORY APPROACH JUSTIFICATION

Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Remarks:

None reported

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

Supernatent from homogenised activated sludge was used as an inoculum.

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

ThOD/L

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The supernatent from the homogenized activated sludge was used as an innoculum. The innoculum was pre adapted to the test material for 14 days, during which the test substance was added incrementally at conc equivalent to 4, 8, and 16 mg carbon/l on days 0, 7 and 12. The targeted microbial level was 10,000 to 100,000 cells/ml. A measured volume of the inoculated mineral medium containing approximately 100 mg/l test substance was stirred continuously in a closed system for 28 days. Test vessels were incubated at 23 ± 1°C.

Reference substance:

benzoic acid, sodium salt

Test performance:

The positive control was degraded by >60% within 3 days.

Key result

Parameter:

% degradation (O2 consumption)

Value:

8.6

Sampling time:

28 d

Results with reference substance:

The reference substance was degraded by >60% within 3 days and 88.8% degraded after 28 days.

Validity criteria fulfilled:

yes

Remarks:

The reference substance achived >60% degradation, therefore, the test was considered valid.

Interpretation of results:

under test conditions no biodegradation observed

Executive summary:

A 28 day aerobic ready biodegradability study (to OECD Guideline No. 301F – Closed Bottle Test) was carried out using an analog of the submission substance (CAS Number 70024-71-4). The effects of the test substance on ready biodegradability were assessed based on the measurement of oxygen uptake using an activated sludge inoculum (from a domestic wastewater plant) that was pre-adapted to the test substance for 14 days. Test performance was evaluated using the reference substance sodium benzoate.

 

This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests.

 

Results Synopsis

 

There was only 8.6% degradation of the test substance after 28 days.

There was 89% degradation of of the reference substance sodium benzoate after 28 days meaning the test was considered valid.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Study meets the criteria for Klimisch code 1 because it followed an internationally recognised method (OECD Guideline 301B) and was carried out to Good Laboratory Practice. However as this study is used in the context of a read across, Klimisch 2 is assigned.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: magnesium long chain alkaryl sulfonate
3. CATEGORY APPROACH JUSTIFICATION
Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Remarks:

None reported

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Activated sludge from domestic wastewater treatment works prepared per test guideline.

Duration of test (contact time):

28 d

Initial conc.:

20 other: mg C/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Triplicate test vessels were used for the test substance, positive control and the control blank. Test vessels were incubated at 20-23°C.

Reference substance:

benzoic acid, sodium salt

Test performance:

The positive control was degraded by >60% within 28 days.

Key result

Parameter:

% degradation (CO2 evolution)

Value:

1.5

Sampling time:

28 d

Remarks on result:

other: With additional sampling on days 2, 4, 7, 10, 14, 17, 21 and 24

Results with reference substance:

89.2% after 28 days.

Validity criteria fulfilled:

yes

Remarks:

The reference substance achieved greater than or equal to 60% degradation, and therefore, the test is considered valid.

Interpretation of results:

under test conditions no biodegradation observed

Executive summary:

A 28 day aerobic ready biodegradability study (to OECD Guideline No. 301B – Modified Sturm Test) was carried out using an analogue of the submission substance (CAS Number 70024-71-4). The effects of the test substance on ready biodegradability were assessed based on the measurement of carbon dioxide evolution using an activated sludge inoculum (from a domestic wastewater plant) that was not pre-adapted to the test substance. Test performance was evaluated using the reference substance sodium benzoate.

 

 This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests.

 

Results Synopsis

 

There was only 1.5% degradation of the test substance after 28 days.

There was 89% degradation of of the reference substance sodium benzoate after 28 days meaning the test was considered valid.

Reference 4

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Study meets the criteria for Klimisch score 1 because it followed an internationally recognised method (OECD Guideline 301B) and was carried out to Good Laboratory Practice. However as this study is used in the context of a read across, Klimisch 2 is assigned.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: Calcium alkaryl sulfonate
3. CATEGORY APPROACH JUSTIFICATION
Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Remarks:

None reported

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Activated sludge from a domestic wastewater treatment plant prepared per test guideline.

Duration of test (contact time):

28 d

Initial conc.:

20 other: mg C/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Triplicate test vessels were used for the test substance, positive control and control blank. Test vessels were incubated at 20-23°C.

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

9.1

Sampling time:

28 d

Remarks on result:

other: With additional sampling on days 1, 3, 5, 7, 10, 13, 17, 20 and 24

Results with reference substance:

86.1% degradation after 28 days.

Validity criteria fulfilled:

yes

Remarks:

The reference substance achieved greater ir equal to 60% degradation, and therefore, the test was considered valid

Interpretation of results:

under test conditions no biodegradation observed

Executive summary:

A 28-day aerobic ready biodegradability study (to OECD Guideline No. 301B – Modified Sturm Test) was carried out using an analogue of the submission substance. The effects of the test substance on ready biodegradability were assessed based on the measurement of carbon dioxide evolution using an activated sludge inoculum (from a domestic wastewater plant) that was not pre-adapted to the test substance. Test performance was evaluated using the reference substance sodium benzoate.

 

This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests.

 

Results Synopsis

 

There was only 9.1% degradation of the test substance after 28 days.

There was 86% degradation of of the reference substance sodium benzoate after 28 days meaning the test was considered valid.

Reference 5

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Study period:

October 3, 2006 - November 29, 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

No report on this study was provided to WRc, therefore, this data has not been reviewed. All data provided in this study record was entered by the data owner.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: Calcium long chain alkaryl sulphonate
3. CATEGORY APPROACH JUSTIFICATION
Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

A fresh sample of activated sludge was colIected from the aeration tank of Shenyang Fairy Ever Sewage Treatment Center. This plant is a main sewage plant in Shenyang for domestic waste treatment. Some of the sample was shaken for 24 hours, and the coarse particles were removed by filtration through a fine sieve and kept aerobic. The sludge was washed in the mineral nutrient medium, suspended to yield a concentration of 3-5g SS/L with dry weight method, and aerated until required thereafter.

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

See attached study report for details

Reference substance:

benzoic acid, sodium salt

Preliminary study:

See attached study report for details

Test performance:

See attached study report for details

Key result

Parameter:

% degradation (CO2 evolution)

Value:

78.6

Sampling time:

28 d

Details on results:

The accumulated percentage biodegradation in activated sludge 1, 4, 5, 6, 8, 10, 13, 15, 17,23,26 and 28 days after treatment were 2.44%, 4.82%, 11.4%, 16.7%, 28.9%, 42.6%, 52.9%, 65.5%, 72.9%, 74.2%, 75.8% and 78.6%. Ten percent degradation had been reached after approximately 5 days and within the 10-day window, biodegradation of the test substance, {CAS# 722503-68-6} had reached the pass level of 60%.

See attached study report for details

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Based on these results and the grading criteria in the Guidelines for the Hazard Evaluation of New Chemical Substances, State Environmental Protection Agency of P.R.C. (HJ/T 154-2004), the test substance, {CAS# 722503-68-6} was concluded as easily (readily) biodegradable in this test condition.

Executive summary:

Based on these results and the grading criteria in the Guidelines for the Hazard Evaluation of New Chemical Substances, State Environmental Protection Agency of P.RC. (HJ/T 154-2004), the test substance, {CAS# 722503-68-6} was concluded as easily (readily) biodegradable in this test condition.

Reference 6

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Study period:

December 16, 2004 to February 25. 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

There were no circumstances which might have affected the reliability of the test results No report on this study was provided to WRc, therefore, this data has not been reviewed. All data provided in this study record was entered by the data owner.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: Calcium long chain alkaryl sulphonate
3. CATEGORY APPROACH JUSTIFICATION
Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Qualifier:

according to guideline

Guideline:

other: Japanese Guidelines

Principles of method if other than guideline:

Japanese Authorities

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

See attached study report for details

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

formulation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

See attached study report for details

Reference substance:

aniline

Preliminary study:

See attached study report for details

Test performance:

See attached study report for details

Key result

Parameter:

% degradation (O2 consumption)

Value:

1

Sampling time:

28 d

Details on results:

On day 28, the average degradability besed on BOD was 1 %, and the average degradability based on residual substance was 1 %.

Key result

Parameter:

BOD5

Value:

76 mg O2/g test mat.

Key result

Parameter:

COD

Value:

20 mg O2/g test mat.

Results with reference substance:

See attached study report for details

See attached study report for details

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The substance is not readily biodegradable.

Reference 7

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

11/10/97 - 1/15/98

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No report on this study was provided to WRc, therefore, this data has not been reviewed. All data provided in this study record was entered by the data owner.

Justification for type of information:

REPORTING FORMAT FOR THE CATEGORY APPROACH
1. HYPOTHESIS FOR THE CATEGORY APPROACH
The read across follows Scenario 5 - Qualitatively and quantitatively similar effects are caused by a common compound, which is formed from all category members (as described in the 2017 Read-Across Assessment Framework document).
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
TARGET: Benzene, tetrapropylene-, distn. residues, sulfonated, sodium salts
SOURCE: Calcium long chain alkaryl sulphonate
3. CATEGORY APPROACH JUSTIFICATION
Linear and non-linear or branched alkylbenzene sulfonates are anionic surfactants with molecules characterized by a hydrophobic (apolar) and a hydrophilic (polar) group. As a group of chemicals, they are generally mixtures of closely related isomers and homologues. Each molecule contains an aromatic ring sulfonated at the para position and attached to either a linear or a branched alkyl chain at any position except the terminal carbons. The sulfonate group is a common functional group present in each of the category members, and is expected to exhibit similar biological activities with little influence from the length of carbon chain. The cation components of the chemicals (e.g. calcium, magnesium, sodium, or barium) are not expected to contribute significantly to the toxicity.
4. DATA MATRIX
See Read Across document attached to CSR

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

mixture of sewage, soil and natural water

Duration of test (contact time):

ca. 28 d

Initial conc.:

10 mg/L

Based on:

formulation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

other: Canola Oil

Preliminary study:

See study report for details

Test performance:

See study report for details

Key result

Parameter:

% degradation (O2 consumption)

Value:

12.5

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

12.5% theoretical CO2 evolved; material not readily biodegradeable

Description of key information

Several biodegradation in water screening tests were found that were performed on read across source substances. These include studies performed according to OECD Guidelines 301D, 301F and 301B. None of these available studies showed ready biodegradation after 28 days.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

Several biodegradation in water screening tests were found that were performed on read across source substances. These include studies performed according to OECD Guidelines 301D, 301F and 301B. None of these available studies showed ready biodegradation after 28 days.

1) A 28-day aerobic ready biodegradability study (to OECD Guideline No. 301D – Closed Bottle Test) was carried out using an analog of the submission substance (CASN 70024-71-4; benzenesulfonic acid C16-C24 alkaryl derivative, calcium salt. The effects of the test substance on ready biodegradability were assessed based on the measurement of chemical oxygen demand (COD) using an activated sludge inoculum (from a domestic wastewater plant) that was not pre-adapted to the test substance. Test performance was evaluated using the reference substances sodium benzoate and aniline. There was only 8% degradation of the test substance after 28 days. There was 97% and 61% degradation of the reference substances sodium benzoate and aniline after 28 days meaning the test was considered valid.

2) A 28-day aerobic ready biodegradability study (to OECD Guideline No. 301F – Closed Bottle Test) was carried out using an analog of the submission substance (CASN 70024-71-4). The effects of the test substance on ready biodegradability were assessed based on the measurement of oxygen uptake using an activated sludge inoculum (from a domestic wastewater plant) that was pre-adapted to the test substance for 14 days. Test performance was evaluated using the reference substance sodium benzoate. This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests. There was only 8.6% degradation of the test substance after 28 days. There was 89% degradation of the reference substance sodium benzoate after 28 days meaning the test was considered valid.

3) A 28-day aerobic ready biodegradability study (to OECD Guideline No. 301B – Modified Sturm Test) was carried out using an analogue of the submission substance (CASN 70024-71-4). The effects of the test substance on ready biodegradability were assessed based on the measurement of carbon dioxide evolution using an activated sludge inoculum (from a domestic wastewater plant) that was not pre-adapted to the test substance. Test performance was evaluated using the reference substance sodium benzoate. This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests. There was only 1.5% degradation of the test substance after 28 days. There was 89% degradation of the reference substance sodium benzoate after 28 days meaning the test was considered valid.

4) A 28-day aerobic ready biodegradability study (to OECD Guideline No. 301B – Modified Sturm Test) was carried out using an analogue of the submission substance (CASN 70024-71-4). The effects of the test substance on ready biodegradability were assessed based on the measurement of carbon dioxide evolution using an activated sludge inoculum (from a domestic wastewater plant) that was not pre-adapted to the test substance. Test performance was evaluated using the reference substance sodium benzoate. This toxicity study is classified as reliable without restriction (Klimisch Code 1) and satisfies the guideline requirement for biodegradation in water-screening tests. There was only 9.1% degradation of the test substance after 28 days. There was 86% degradation of the reference substance sodium benzoate after 28 days meaning the test was considered valid.