1,8-dihydroxy-4-nitro-5-(phenylamino)anthraquinone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Test substance: Terasil Blau GRN Isomerengemisch roh feucht
Code: FAT 92504/A
Batch-No.: 1/95
Appearance: solid blue
Solubility (g/l): <0.1
Storage: room temperature
Expiration date: 04/1999

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

None

Test organisms (species):

Daphnia magna

Details on test organisms:

None

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

21 °C

pH:

7.6 - 7.8

Dissolved oxygen:

97 - 99 % at the end of the exposure

Nominal and measured concentrations:

0.1, 1, 10 and 100 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

1 out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48-hour exposure period. Apart from this, no mortality was observed at any other cocentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups.

Results with reference substance (positive control):

None

Validity criteria fulfilled:

yes

Conclusions:

The 48h EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.

Executive summary:

The short-term toxicity of FAT 92504/A to aquatic invertebrates was evaluated in a study conducted according to OECD Guideline 202. In this study, groups of 10 daphnids were exposed to the test concentrations of 0.1, 1, 10 and 100 mg/L for 48 hours. The daphnids were also exposed to blank and vehicle groups. The observations of mobility were recorded to determine the toxicity of the substance. 1 out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48-hour exposure period. Apart from this, no mortality was observed at any other concentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups. Hence, the 48h EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.

Description of key information

Currently, study for assessment of toxicity potential of Disperse Blue 077 to aquatic invertebrates is not available. However, the short-term toxicity of the structurally similar substance, Disperse Blue 054/077 (FAT 92504/A) to aquatic invertebrates was evaluated in a study conducted according to OECD Guideline 202. In this study, groups of 10 daphnids were exposed to the test concentrations of 0.1, 1, 10 and 100 mg/L for 48 hours. The daphnids were also exposed to blank and vehicle groups. The observations of mobility were recorded to determine the toxicity of the substance. 1 out of 10 daphnids at the concentration of 10 mg/L was found dead at the end of 48-hour exposure period. Apart from this, no mortality was observed at any other concentrations of 0.1, 1 and 100 mg/L as well as blank or vehicle groups. Hence, the 48h EC50 of FAT 92504/A to daphnids was determined to be >100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L