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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [SPEC][/SPEC][SYN]Nitrososilane[/SYN]
- IUPAC Name:
- [SPEC][/SPEC][SYN]Nitrososilane[/SYN]
- Reference substance name:
- [CS]000000419807[/CS]
- IUPAC Name:
- [CS]000000419807[/CS]
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Test item: Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester
Batch Number: 12/34#
Content: 93.3 area-%
CAS RN: 1195231-94-7
Sum formula: C20H35N3O6Si
Molecular weight: 441 g/mol
Appearance: Solid, green powder
Water solubility: 70 mg/L
Stability under test conditions: At pH 7: ca. 40 h half life
Expiry date: 2013-09-17
Recommended storage: Room temperature, avoid moisture, keep dark
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.022 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.079 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.069 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: with 95% confidence limits=0.0326-0.137
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.134 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: with 95% confidence limits=0.0743-0.208
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.401 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: with 95% confidence limits=0.293-0.565
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.022 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.079 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.035 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: with 95% confidence limits = <0.0218-0.0623
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.043 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: with 95% confidence limits=0.0236-0.0645
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.059 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: with 95% confidence limits=0.0320-0.0725
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values (geometric mean measured test item concentrations): The EC50-values with 95 % confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 0.401 (0.293 – 0.565) mg/L and 0.0594 (0.0320 – 0.0725) mg/L, respectively. The NOEC-value for both inhibitions of growth rate and yield after 72 hours was 0.0218 mg/L.
All effect values are given based on geometric mean measured test item concentrations. - Executive summary:
The toxicity of Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester (CAS 1195231-94-7) to the unicellular freshwater green alga Pseudokirchneriella subcapitatawas determined according to the principles of OECD 201 at Dr.U.Noack-Laboratorien in 31157 Sarstedt, Germany from 2013-07-30 to 2013-08-05, with the definitive exposure phase from 2013-07-30 to 2013-08-02. The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 5297 cells/mL. Based on a preliminary test 5 concentration levels were tested in a geometrical series with a dilution factor of (nominal): 0.100 - 0.316 - 1.00 - 3.16 - 10.0 mg/L corresponding to the geometric mean measured concentrations: 0.0218 - 0.0785 - 0.271 - 0.823 - 3.71 mg/L. Three replicates were tested for each test item concentration and six replicates for the control. The environmental conditionswere within the acceptable limits.
The concentrations of Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl esterwere analytically verified by LC-MS/MS at the test start and the test end of all test concentrations and the control.
The measured concentrations of Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl esterat start of the exposure were in the range of 52 to 58 % and after 72 hours in the range of 8 to 25 % of the nominal values. Therefore, all effect values given are based on the geometric mean measured concentrations after 0 and 72 hours of the test itemCarbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester.
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