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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2013-08-01 to 2013-09-27
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study and GLP

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Test material form:
solid: particulate/powder
migrated information: powder
Details on test material:
Test Item: Carbamic acid, N-(3-(triethoxysiIypropyI2-(ethyI-(4- nitrosophenyl) amino) ethyl ester
Batch number: 12/34#
CAS nurnber: 1195231-94-7
Content: 93.3 %
Sum formula: C20H35N3O6Si
Molecular weight: 441 g/mol
Appearance: Solid/powder/green
Water solubility: 70 mg/L
TOC*: 54.4 %
Expiry date: 2013-09-17
Recommended storage: Room temperature, protected from Iight, in desiccator.

*TOC calculated at test facility

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, non-adapted
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
20 mg/L
Based on:
act. ingr.

Results and discussion

% Degradationopen allclose all
% degradation (CO2 evolution)
Sampling time:
28 d
Remarks on result:
other: Test item 1
% degradation (CO2 evolution)
Sampling time:
28 d
Remarks on result:
other: Test item 2
Details on results:
The total amount of CO2 produced in 28 days was analysed by titration in 12 measurements. The 28 d-values are shown in comparison to the readily degradable functional control in summarized form in Table 2.

Any other information on results incl. tables

Table 2. CO2-Production and Biodegradation after 28 Days

 CO2-Production  Functional Control  Test Item 1  Test Item 2 Toxicity Control test item +reference item
 Net [mg/3 L] [mg/L] 89.529.8  18.56.2  7.12.4  113.637.9 
 Theor. [mg/3 L] [mg/L] 127.842.6  12040  12040  247.882.6 
 Degradation [%]  70  15  6  46

Applicant's summary and conclusion

Validity criteria fulfilled:
Interpretation of results:
other: not readily biodegradable
Under the test conditions the test item is classified as not readily biodegradable in the 10-day-window and within 28 days.
Executive summary:

The ready biodegradability of the test item Carbamic acid, N-(3-(,triethoxysilypropyI)2-(ethyI-(4- nitrosophenyl) amina) ethyl ester (CAS number 1195231-94-7) was determined with a non-adapted activated sludge over a test period of 28 days in the Modified Sturm Test.

The study was conducted from 2013-08-08 to 2013-09-06 according to OECD 301 B at DR.U.NOACK- LABORATORIEN.

The test item was tested at a concentration of 20.0 mg/L with 2 replicates, corresponding to a carbon content (TOC) of 10.9 mg C/L in the test vessels. The test vessels were incubated at low Iight conditions and at a temperature of 21.0 -24.0 °C.

The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2 produced by the respiration of bacteria. The degradation was stopped on day 28 by acidificatian of the test solutions. The last titration was made on day 29, after residual CO2 had been purged from the test solutions over a period of 24 hours. The percentage CO2 production was calculated in relation to the theoretical CO2 production (ThCO2) of the test item. The biodegradation was calculated for each titration time.

To check the activity of the test system, sodium benzoate was used as functional control. The percentage degradation of the functional control reached the pass level of 60 % after 10 days and a biodegradation af 70% after 28 days.

In the toxicity control containing both test and reference item a biodegradation of 39 % was determined within 14 days and it came to 46 % after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control.

The biodegradation of the test item is shown graphically in Figure 1 in comparison to the readily degradable functional control and the toxicity control. The biodegradation was slowly and the mean 10 % level (beginning of biodegradation) was only reached on day 28.