2-[alkyl(4-nitrosoaryl)amino]alkyl [3-(trialkoxysilyl)propyl]carbamate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[SPEC][/SPEC][SYN]Nitrososilane[/SYN]

IUPAC Name:

[SPEC][/SPEC][SYN]Nitrososilane[/SYN]

Constituent 2

Reference substance name:

[CS]000000419807[/CS]

IUPAC Name:

[CS]000000419807[/CS]

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Test item: Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester
Batch Number: 12/34#
Content: 93.3 area-%
CAS RN: 1195231-94-7
Sum formula: C20H35N3O6Si
Molecular weight: 441 g/mol
Appearance: Solid, green powder
Water solubility: 70 mg/L
Stability under test conditions: At pH 7: ca. 40 h half life
Expiry date: 2013-09-17
Recommended storage: Room temperature, avoid moisture, keep dark

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1.8 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

3.66 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

3.21 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: with 95% confidence limits=3.03-3.82

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.7 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: with 95% confidence limits=3.59-3.82

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the geometric mean measured concentrations of the test item Carbamic acid, N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester the 48 h-EC50 for Daphnia magna was 3.70 mg/L (95 % confidence limits: 3.59 – 3.82 mg/L).
The NOEC after 48 h was 1.80 mg/L. The LOEC after 48 h was 3.66 mg/L.

Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item Carbamic acid,N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl ester (CAS1195231 -94 -7) were determined according to OECD 202 (2004) from 2013-07-30 to 2013-08-01 at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str.1, 31157 Sarstedt, Germany.

 

The study was conducted under semi-static conditions over a period of 48 h with 5 concentrations of the test item Carbamic acid,N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl esterin the range of 0.600 to 14.1 mg/L, prepared with dilution water in a geometric series with a separation factor of 2.2.

The test item Carbamic acid,N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl esteris a green powder and has a water solubility of approximately 70 mg/L. Therefore, the test concentrations were selected not exceeding the solubility limit. However, obviously the solubility of the test item in the dilution water (ISO test water, according to OECD 202, Annex 3) under test conditions is lower than 70 mg/L. During the preparation of the stock solution of 14.1 mg test item/L, undissolved test item was observed sticking on the magnetic stir bar. The tested solutions were clear and showed a concentration related yellow colour.Twenty daphnids were exposed to each concentration level and the control.

 

The concentrations of the test item Carbamic acid,N-(3-(triethoxysilyl)propyl)2-(ethyl-(4-nitrosophenyl) amino) ethyl esterwereanalytically verified by LC-MS/MS at the start of the exposure intervals (0 and 24 h) and at the end of the exposure intervals (24 and 48 h) in all concentration levels and the control. Details of the analytical method are presented in part15.
The measured concentrations of the test item at the start of the exposure intervals (0 and 24 h) were in the range of 57 to 89 % of the nominal values. At the end of the exposure intervals (24 and 48 h), the measured concentrations of the test item were in the range of 40 to 66 % of the nominal values. The geometric mean measured concentrations of the test item were calculated to be: 0.403 – 0.881 – 1.80 – 3.66 – 8.65 mg/L.

 

The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 and 24 h) and at the end of the exposure intervals (24 and 48 h), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.