Methylammonium chloride

Endpoint summary

Description of key information

LD50 was observed at the dose level of 1600 mg/kg bw in range of1600 to 3200 mg/kg.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data from peer reveiw publication

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To evaluate the acute toxicity of Methylamine hydrochloride in rats.

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): Methylamine hydrochloride (methanaminium chloride)
- Molecular formula (if other than submission substance): CH5N.Cl H
- Molecular weight (if other than submission substance): 67.5184 g/mole
- Substance type: Organic

Species:

rat

Strain:

not specified

Sex:

not specified

Route of administration:

oral: unspecified

Vehicle:

not specified

Details on oral exposure:

No data available.

Doses:

1600 to 3200 mg/kg

No. of animals per sex per dose:

No data available.

Control animals:

not specified

Details on study design:

No data available.

Statistics:

No data available.

Preliminary study:

No data available.

Sex:

not specified

Dose descriptor:

LD50

Effect level:

1 600 mg/kg bw

Based on:

test mat.

95% CL:

1 600 - 3 200

Remarks on result:

other: 50 % mortality observed

Mortality:

50 % mortality was observed.

Clinical signs:

other: No data available.

Gross pathology:

No data available.

Other findings:

No data available.

Interpretation of results:

Toxicity Category IV

Conclusions:

LD50 was considered to be 1600 mg/kg for Methylamine hydrochloride in rats by oral administration.

Executive summary:

In a acute oral toxicity study, rats were treated with methanaminium chloride orally. The LD50 was observed at the dose level of 1600 mg/kg bw in the range of 1600 to 3200 mg/kg.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

1 600 mg/kg bw

Quality of whole database:

Data is Klimisch 2 and from peer-reviewed journal

Acute toxicity: via inhalation route

Endpoint conclusion

Endpoint conclusion:

no study available

Acute toxicity: via dermal route

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Acute oral toxicity:

In different studies, Methanaminium chloride has been investigated for acute oral toxicity to a greater or lesser extent. Often are the studies based on in vivo experiments in rodents, i.e. most commonly in rats for Methanaminium chloride along with the study available on structurally similar read across substance Methylamine (CAS: 74-89-5). The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

In a study by Kinney et al (Inhalation Toxicology, 2:29-39, 1990), rats were administered methanaminium chloride orally to gauge the acute oral toxicity. The LD50 was observed at the dose level of 1600 mg/kg bw in range of1600 to 3200 mg/kg.

In another prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the acute oral toxicity was estimated for methanaminium chloride. The LD50 was estimated to be 1296 mg/kg bw when Sprague-Dawley male and female rats were orally exposed with 4- methanaminium chloride.

Also it is further supported experimental data by MAK Collection (MAK Value Documentation, 146–153, 2012) on structurally similar read across substance Methylamine (CAS: 74-89-5), rats were treated with Methylamine orally as 40 % solution. LD50 was considered to be 698 mg/kg bw (547 – 1007 mg/kg) when rat were treated with Methylamine orally.

Thus, based on the above predictions and study on Methanaminium chloride and its read across substances and by conceding the weight of evidence, it can be concluded that LD50 value is less than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Methanaminium chloride can be classified as “Category IV” of acute oral toxicity. 

Justification for classification or non-classification

Based on the above predictions and study on Methanaminium chloride and its read across substances and by conceding the weight of evidence, it can be concluded that LD50 value is less than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Methanaminium chloride can be classified as “Category IV” of acute oral toxicity.