Registration Dossier
Registration Dossier
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EC number: 205-275-2 | CAS number: 137-05-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline; only short abstract
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Ames test, no guideline cited
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Mecrilate
- EC Number:
- 205-275-2
- EC Name:
- Mecrilate
- Cas Number:
- 137-05-3
- Molecular formula:
- C5H5NO2
- IUPAC Name:
- methyl 2-cyanoprop-2-enoate
- Test material form:
- liquid
- Details on test material:
- Test of five cyanoacrylates
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 100, TA 1535, TA 1537
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 20, 60, 180, 540, 1620 µg per plate
- Vehicle / solvent:
- Acetone
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- - Plate incorporation test with Salmonella typhimurium TA 100 and TA 1535 (standard conditions without metabolic activation).
- Toxitcity test with Salmonella typhimurium TA 1537 without metabolic activation.
- Application volume: 50 µL per plate. - Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium, other: TA100, TA1535
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
Any other information on results incl. tables
Methylcyanoacrylate did not induce an increase of the revertant rates in the plate incorporation test with Salmonella typhimurium TA 100 and TA 1535 without metabolic activation and is therefore considered not mutagenic under the conditions of this test.
In the toxicity test with Salmonella typhimurium TA 1537 without metabolic activation a dose related inhibition of the colony growth up to 43% was observed.
Methylcyanoacrylate showed the the highest bacteriotoxic potential in comparison to the other tested cyanoacrylates.
Applicant's summary and conclusion
- Conclusions:
- Under the conditions of this test, methylcyanoacrylate was not mutagenic.
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