Mecrilate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study meets generally accepted scientific principles, but was not conducted in compliance with GLP or an OECD testing guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

no

Remarks:

prior to GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Mecrilate

EC Number:

205-275-2

EC Name:

Mecrilate

Cas Number:

137-05-3

Molecular formula:

C5H5NO2

IUPAC Name:

methyl 2-cyanoprop-2-enoate

Details on test material:

- Name(s): Methyl 2-cyanoacrylate, Mecrilate, 2-Propenoic acid, 2-cyano-, methyl ester
- CAS 137-05-3
- Appearance: clear, colorless glue
- Purity: 88.8%

Test animals

Species:

rat

Strain:

other: Albino

Sex:

male

Details on test animals or test system and environmental conditions:

6 male albino rats fasted for 18 hours prior to dosing.
The initial body weights ranged from 195 to 221 grams.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

The animals were observed during the day of dosing and daily thereafter for 14 days.
Decedents during the study were examined for gross lesions.

Statistics:

no

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

> 4 440 mg/kg bw

Based on:

act. ingr.

Remarks on result:

other: 88.8 % ai

Mortality:

None (0/6)

Clinical signs:

other: Pilo-erection, lethargy

Gross pathology:

Caecum slightly enlarged , blood vessels of stomach, caecum and intestines were injected; large hardened mass of the test material of approximately the same size and shape of the stomach was found in the stomach of each animal sacrificed. However, no ulcers were observed in the stomach.

Other findings:

None

Any other information on results incl. tables

RESULTS

Onset of signs (S):

0 - 4 h	4 - 8 h	8 - 24 h
6 S	-	-

Mortaliy (M) and Recovery (R):

day 2	day 3	day 4	day 5	day 6	days 7 - 14
-	-	-	3 R	3 R	-

Caecum slightly enlarged, blood vessels of stomach. caecum and intestines were injected; large hardened mass of the test material of approximately the same size and shape of the stomach was found in the stomach of each animal sacrificed, however, no ulcers were observed in the stomach and no loss of body weight occurred in any of the animals during the 14-day period of observation.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 was found to be greater than 5000 mg/kg bw (greater than 4400 mg/kg bw relating to methyl 2-cyanoacrylate). In conclusion, the test material can be considered as practically nontoxic.

Executive summary:

In a limit test with 6 male Albino rats, 5000 mg/kg bw of the Loctite glue "150 Super Bonder" was administered by gavage. All animals exhibited signs of intoxication (pilo-erection, lethargy), which were reversible within 5-6 days. No mortality was observed during the 14 -day observation period. Therefore, the LD50 for the tested material has been identified as > 5000 mg/kg bw (corresponding to 4400 mg/kg of the active ingredient methyl 2 -cyanoacrylate).