Mecrilate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Unsuitable method (concentration too high, time period too low)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

no guideline followed

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2-cyanoacrylate

EC Number:

230-391-5

EC Name:

Ethyl 2-cyanoacrylate

Cas Number:

7085-85-0

Molecular formula:

C6H7NO2

IUPAC Name:

ethyl 2-cyanoacrylate

Details on test material:

- Substance name: Ethyl cyanoacrylate
- CAS 7085-85-0
- Appearance: clear, colorless liquid
- Purity: 99.9 %

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

ANIMALS:
Five male and five female Wistar derived albino rats, weighing between 200-300g, from Royalhart Farms.

ENVIRONMENTAL CONDITIONS:
During the test period the animals were individually housed in stainless steel wire bottomed cages in an environmentally controlled room with a
12 hour Iight/dark cycle. Feed (Fisher Rat Pellets) and water were provided ad-Iibitum after dosing.

Administration / exposure

Route of administration:

inhalation: mist

Type of inhalation exposure:

not specified

Vehicle:

air

Details on inhalation exposure:

During the first 30 minutes the test material (1.9 g total) was nebulized into the inhalation chamber after being warmed in a vesseI submerged in water at 35-37°C. The water bath temperature was decreased to 25°C during the remainder (30 minutes) of the exposure period.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

gravimetrical determination

Duration of exposure:

1 h

Concentrations:

1.9 g (approx. 21.11 mg/L/h)

No. of animals per sex per dose:

5

Control animals:

no

Statistics:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

< 21.22 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

1 h

Mortality:

Total mortality was 70 %. All mortalities occurred within 4 days post dose.

Clinical signs:

other: During the exposure period the rats were extremely irritable and showed signs of severe respiratory stress, eye irritation and skin irritation. Several animals also suffered nasal and ocular bleeding during the exposure period.

Body weight:

see " Any other information on results"

Gross pathology:

Gross necropsies were performed on all mortalities. Pulmonary hemorrhage, intestinal hemorrhage and/or splenic hemorrhage were observed in all pathological examinations.

Any other information on results incl. tables

INDIVIDUAL RESULTS

Animal	Sex	BW initial	BW final	Mortality (day)	Autopsy
5973	male	285	263	1	PH, SH, IH
5974	male	290	257	2	PH, SH, IH
5975	male	243	245	-	NA
5976	male	299	243	2	PH, SH, IH
5977	male	266	213	4	PH, IH
5978	female	248	204	4	PH, IH, SH
5979	female	225	172	3	PH, IH, SH
5980	female	232	241	-	NA
5981	female	245	201	2	PH, SH, slight IH
5982	female	256	277	-	NA

PH = Pulmonary hemorrhage

SH = Splenic hemorrhage

IH = Intestinal hemorrhage

NA = not applicable

Applicant's summary and conclusion

Interpretation of results:

other: no final interpretation possible due to unsuitable test method

Conclusions:

The LC50 of Ethyl cyanoacrylate is < 21.11 mg/L/h (nominal) unser the conditions of this study.