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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

Currently viewing:

Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No GLP compliance

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 406 (Skin Sensitisation)
Qualifier:
according to guideline
Guideline:
other: EEC 84/449/EEC
GLP compliance:
no
Type of study:
guinea pig maximisation test

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4,6-trichloro-1,3,5-triazine
EC Number:
203-614-9
EC Name:
2,4,6-trichloro-1,3,5-triazine
Cas Number:
108-77-0
Molecular formula:
C3Cl3N3
IUPAC Name:
2,4,6-trichloro-1,3,5-triazine
Details on test material:
Cyanuric chloride, purity >95%.

In vivo test system

Test animals

Species:
guinea pig
Strain:
Hartley
Sex:
female
Details on test animals and environmental conditions:
age 9-10 weeks
weight 270-410 g

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Route:
intradermal and epicutaneous
Vehicle:
polyethylene glycol
Concentration / amount:
Intradermal induction (0.01% in polyethylene glycol)
topical induction (2% in polyethylene glycol)
challenge (1% in polyethylene glycol)
Challenge
Concentration / amount:
Intradermal induction (0.01% in polyethylene glycol)
topical induction (2% in polyethylene glycol)
challenge (1% in polyethylene glycol)
No. of animals per dose:
12 females/treatment
8 females for controls
Details on study design:
Procedure: As per OECD 406: Intradermal induction (0.01% in polyethylene glycol) on day 1, topical induction on day 8 (2% in polyethylene glycol), challenge on day 22 (1% in polyethylene glycol), skin reading after 24 and 48 h (day 24 and 25).
Observations:
Skin reactions 24 and 48 hours after the challenge exposure.
Mortality/clinical signs (frequency not indicated).
Body weight on day 2 and weekly thereafter.
Positive control substance(s):
yes
Remarks:
p-phenylene-diamine

Results and discussion

Positive control results:
Not reported.

In vivo (non-LLNA)

Resultsopen allclose all
Group:
test chemical
No. with + reactions:
0
Total no. in group:
12
Clinical observations:
mortality/clinical signs
Remarks on result:
other: Group: test group. No with. + reactions: 0.0. Total no. in groups: 12.0. Clinical observations: mortality/clinical signs.
Group:
positive control
No. with + reactions:
0
Total no. in group:
8
Clinical observations:
mortality/clinical signs
Remarks on result:
other: Group: positive control. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: mortality/clinical signs.
Group:
test chemical
No. with + reactions:
0
Total no. in group:
12
Clinical observations:
Body weight
Remarks on result:
other: Group: test group. No with. + reactions: 0.0. Total no. in groups: 12.0. Clinical observations: Body weight.
Group:
positive control
No. with + reactions:
0
Total no. in group:
8
Clinical observations:
body weight
Remarks on result:
other: Group: positive control. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: body weight.
Hours after challenge:
24
Group:
test chemical
No. with + reactions:
12
Total no. in group:
12
Clinical observations:
No. with erythema/oedema score > 0 (24/48h)
Remarks on result:
other: . Hours after challenge: 24.0. Group: test group. No with. + reactions: 12.0. Total no. in groups: 12.0. Clinical observations: No. with erythema/oedema score > 0 (24/48h).
Hours after challenge:
24
Group:
positive control
No. with + reactions:
0
Total no. in group:
8
Clinical observations:
No. with erythema/oedema score > 0 (24/48h)
Remarks on result:
other: . Hours after challenge: 24.0. Group: positive control. No with. + reactions: 0.0. Total no. in groups: 8.0. Clinical observations: No. with erythema/oedema score > 0 (24/48h).

Any other information on results incl. tables

No more available data on the results.

Applicant's summary and conclusion

Interpretation of results:
sensitising
Remarks:
Migrated information
Conclusions:
Sensitising
Executive summary:

The cutaneous sensitising properties of Cyanuric chloride were tested in the guinea-pig (maximization test). The number of animals was 12 females/treatment and 8 females for controls. The procedure was designed as per OECD 406. Skin reactions 24 and 48 hours after the challenge exposure, mortality/clinical signs and body weight were observed. P-phenylene-diamine was used as a positive control. Cyanuric chloride was found to be sensitising.