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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
genetic toxicity in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: No data on GLP and OECD guidelines compliance

Data source

Reference
Reference Type:
publication
Title:
Cyanuric Chloride Has No Genotoxic and Mutagenic Properties in Bacteria and Bone Marrow Cells
Author:
Wyszynska K, Przybojewska B, Spiechowicz E, Chwialkowska-Liro W, Dziubaltowska E, Rydzynski K
Year:
1994
Bibliographic source:
International Journal of Occupational Medicine and Environmental Health, Vol. 7, No. 3, pages 281-289, 1994

Materials and methods

Principles of method if other than guideline:
No data
GLP compliance:
not specified
Type of assay:
other: sister chromatid exchanges (SCEs) and polychromatic erythrocyte (PCE) micronuclei.

Test material

Constituent 1
Chemical structure
Reference substance name:
2,4,6-trichloro-1,3,5-triazine
EC Number:
203-614-9
EC Name:
2,4,6-trichloro-1,3,5-triazine
Cas Number:
108-77-0
Molecular formula:
C3Cl3N3
IUPAC Name:
2,4,6-trichloro-1,3,5-triazine

Test animals

Species:
mouse
Strain:
Balb/c
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Frequency of treatment:
One injection
Post exposure period:
23, 30, or 72 hours after dosing the animals were killed.
Doses / concentrations
Remarks:
Doses / Concentrations:
up to 32mg/kg
Basis:
nominal conc.
Control animals:
not specified

Examinations

Tissues and cell types examined:
The bone marrow was flushed out and scored for sister chromatid exchanges (SCEs) or polychromatic erythrocyte (PCE) micronuclei. . The PCE/normochromatic erythrocyte (NCE) ratios were determined.

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Additional information on results:
Cyanuric-chloride did not induce SCEs or micronucleated PCEs at any time point in mice. The PCE/NCE ratio was significantly decreased at all sampling times.
Cyanuric-chloride has not demonstrated genotoxicity in murine bone marrow SCE and PCE micronuclei assays. The significant decrease in PCE/NCE ratio in the micronucleus assay may indicate a possible cytostatic effect.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
No genotoxic
Executive summary:

The genotoxic properties of cyanuric-chloride were studied in-vivo. Male and female mice were injected intraperitoneally with up to 32mg/kg cyanuric-chloride. Mice were killed 23, 30 or 72 hours later and the femurs were removed. The bone marrow was flushed out and scored for sister chromatid exchanges (SCEs) or for polychromatic erythrocyte (PCE) micronuclei. The PCE/normochromatic erythrocyte (NCE) ratios were determined. Cyanuric-chloride did not induce SCEs or micronucleated PCEs at any time point in mice. The PCE/NCE ratio was significantly decreased at all sampling times. The authors conclude that cyanuric-chloride has not demonstrated genotoxicity in murine bone marrow SCE and PCE micronuclei assays. The significant decrease in PCE/NCE ratio in the micronucleus assay may indicate a possible cytostatic effect.