2-aminoethanol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1966

Report date:

1966

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Principles of method if other than guideline:

BASF-Test

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-aminoethanol

EC Number:

205-483-3

EC Name:

2-aminoethanol

Cas Number:

141-43-5

Molecular formula:

C2H7NO

IUPAC Name:

2-aminoethanol

Test material form:

liquid

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.65-2.89 kg
- Diet: ad libitum
- Water: ad libitum

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: adjacent eye (physiological NaCl-solution)

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 mL

Duration of treatment / exposure:

not applicable (tests substance was not washed out)

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: none

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

24/48/72 h

Score:

3

Max. score:

4

Reversibility:

not reversible

Results 2

Irritation parameter:

cornea opacity score

Basis:

animal #2

Time point:

24/48/72 h

Score:

3

Max. score:

4

Reversibility:

not reversible

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #3

Time point:

24/48/72 h

Score:

3

Max. score:

4

Reversibility:

not reversible

Results 4

Irritation parameter:

conjunctivae score

Remarks:

redness

Basis:

animal #1

Time point:

24/48/72 h

Score:

2

Max. score:

3

Reversibility:

not reversible

Results 5

Irritation parameter:

conjunctivae score

Remarks:

redness

Basis:

animal #2

Time point:

24/48/72 h

Score:

0.66

Max. score:

3

Reversibility:

fully reversible

Results 6

Irritation parameter:

conjunctivae score

Remarks:

redness

Basis:

animal #3

Time point:

24/48/72 h

Score:

2

Max. score:

3

Reversibility:

fully reversible

Results 7

Irritation parameter:

chemosis score

Basis:

animal #1

Time point:

24/48/72 h

Score:

0.66

Max. score:

4

Reversibility:

fully reversible

Results 8

Irritation parameter:

chemosis score

Basis:

animal #2

Time point:

24/48/72 h

Score:

1.33

Max. score:

4

Results 9

Irritation parameter:

chemosis score

Basis:

animal #3

Time point:

24/48/72 h

Score:

2

Max. score:

4

Reversibility:

fully reversible

Results 10

Irritation parameter:

iris score

Basis:

animal #1

Time point:

24/48/72 h

Score:

1.3

Max. score:

3

Results 11

Irritation parameter:

iris score

Basis:

animal #2

Time point:

24/48/72 h

Score:

0

Max. score:

3

Results 12

Irritation parameter:

iris score

Basis:

animal #3

Time point:

24/48/72 h

Score:

1.3

Max. score:

3

Irritant / corrosive response data:

Chemical burnings, scars on upper eyelid, purulent secretion, bloody discharge, conjunctival bleedings, iritis, staphyloma, ciliar injections.

Any other information on results incl. tables

 

Readings	Animal	Cornea		Iris	Conjunctiva			Additional findings
		op	ar		red	ch	di
1 h	1	1	-	0	0	0	-	cornea black-red coloured, bloody secretion, constricted pupil, strong secretion
	2	3	-	0	0	0	-	cornea black-grey coloured, bloody secretion, constricted pupil, strong secretion
	3	3	-	0	0	0	-	cornea brown-red coloured, chemical burnings, brown-red secretion
24 h	1	3	-	0	2	1	-	chemical burnings
	2	3	-	0	1	2		chemical burnings
	3	3	-	0	0	2	-	cornea brown-red coloured, chemical burnings, brown-red secretion
48 h	1	3	-	2	2	1	-	crust formation (eyelids)
	2	3	-	-	1	2	-	chemical burning of the eyeball and eyelid
	3	3	-	2	0	2	-	purulent secretion
72 h	1	3	-	2	2	0	-	purulent secretion
	2	3	-	0	0	0	-	purulent secretion, conjunctiva covered with purulent fur
	3	3	-	2	0	2	-	purulent secretion,
8 d	1	3	-	2	2	0	-	purulent secretion
	2	3	-	0	0	0	-	loss of hair
	3	3	-	2	0	0	-	Staphyloma, ciliar injections, conjunctiva white and chemically burned, scar on upper eyelid
Mean 24 -72 h	1	3	-	1.33	2	0.66	-
	2	3	-	0	0.66	1.33	-
	3	3	-	1.33	0	2	-
Mean 24-72 h	1-3	3	-	0.88	0.89	1.33	-

- endpoint not addressed

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria