2-aminoethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in mammalian cells

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

No details on the concentrations used

Data source

Reference

Reference Type:

publication

Title:

Inhibition of Metabolic Cooperation in Chinese Hamster V79 Cells by Various Organic Solvents and Simple Compounds

Author:

Chen TH, et al.

Year:

1984

Bibliographic source:

Cell Biol. Toxicol. 1 (1):155- 171

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Method described in Trosko et al., 1984 (In Handbook of Carcinogen Testing) is based on metabolic cooperation between 6-thioguanine sensitive (HGPRT+) cells and 6-thioguanine resistant (HGPRT-) cells in vitro.

GLP compliance:

no

Type of assay:

in vitro mammalian cell gene mutation test using the Hprt and xprt genes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test material form:

liquid

Method

Species / strain

Species / strain / cell type:

Chinese hamster lung fibroblasts (V79)

Details on mammalian cell type (if applicable):

- Type and identity of media: Modified Eagle's medium
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: yes
- Periodically checked for karyotype stability: no data
- Periodically "cleansed" against high spontaneous background: no data

Additional strain / cell type characteristics:

other: with and without HGPRT deficiency

Metabolic activation:

without

Test concentrations with justification for top dose:

not indicated

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 12-tetra-decanoyl-1-phorbol-13-acetate (TPA)

Results and discussion

Test results

Key result

Species / strain:

Chinese hamster lung fibroblasts (V79)

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid