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EC number: 205-483-3 | CAS number: 141-43-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, no data on GLP, adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity Test Data of Existing Chemical Substances, January 1996
- Author:
- JETOC
- Year:
- 1 996
- Bibliographic source:
- Japan Chemical Industry Ecology-Toxicology & Information Center, Japan
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Qualifier:
- equivalent or similar to
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- This test has been held according to the Notification stipulating the standard provided by the Minister of Labour based on the Industrial Safety and Health Law Article 57-2 Paragraph 1 (Notification No.77 of the Ministry of Labour, dated September 1, 1988) and related Notice (No.603, dated September 16, 1988) .
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): 2-Aminoethanol
- Supplier: Tokyo Kasei Kogyo Co. Ltd.
- Analytical purity: extra pure reagent
- Lot. No. AQ01
Method
- Target gene:
- his
Species / strainopen allclose all
- Species / strain / cell type:
- other: TA98, TA 100, TA 102, TA 104, TA 1535, TA 1537 and TA1538
- Species / strain / cell type:
- other: WP2uvrA and WP2uvrA/pKM101
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 (Sodium phenobarbital and 5,6-benzofravone-induced)
- Test concentrations with justification for top dose:
- 50 - 5000 µg/plate
- Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see below
- Details on test system and experimental conditions:
- The preincubation procedure was performed as described previously (Matsushima et al., 1980). The test compound dissolved in 0.05 or 0.1 ml of solvent was supplemented with 0.5 ml of S9 mix (metabolic activation method) or 0.1M phosphate buffer pH 7.4 (direct method) and 0.1 ml of tester strains which had been cultured in nutrient broth. The mixture was incubated for 20 min at 37°C, then rapidly mixed with 2 ml of molten top agar containing 0.05 µmol/ml of L-histidine and biotin for the Salmonella test. In the E. coli test 0.05 µmol/ml of L-tryptophan was used instead of L-histidine and biotin. Then the top agar mixture was rapidly poured onto a 30 ml of Vogel -Bonner minimal agar plate. All plates were incubated for 48 hours at 37°C and the numbers of revertant colonies were scored.
- Evaluation criteria:
- Two-hold rule criteria was used for data evaluation (Ames et al., 1975). The chemicals are considered to be mutagenic when a dose-related increase in revertant colony count is observed and the number of revertant colonies per plate with the test substance is more than twice that of the negative control (solvent control) and when a reproducibility of test result is observed. Mutagenic potency was calculated by following equation and maximum value of mutagenic potency was expressed as a specific acitivity on the data sheet: mutagenic potency (induced revertants / mg test substance) _(number of induced revertants on the dose X - number of revertant on the solvent control) = mg of test chemical on the dose X .
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: TA98, TA 100, TA 102, TA 104, TA 1535, TA 1537 and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >=2000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: WP2uvrA and WP2uvrA/pKM101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >=2000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
control values (mean ± SD):
Tester strain | Solvent control | positive control | ||
- S9 | + S9 | - S9 | + S9 | |
TA100 | 138 ± 27 | 139 ± 29 | 728 ± 196 | 1011 ± 210 |
TA1535 | 14 ± 5 | 13 ± 4 | 300 ± 81 | 255 ± 70 |
TA98 | 20 ± 8 | 26 ± 7 | 413 ± 82 | 404 ± 118 |
TA1538 (2NF) | 16 ± 3 | 23 ± 5 | 376 ± 79 | 556 ± 216 |
TA1538 (4NQO) | 16 ± 3 | 23 ± 5 | 343 ± 34 | 556 ± 216 |
TA1537 | 8 ± 2 | 11 ± 4 | 497 ± 254 | 196 ± 70 |
TA102 | 260 ± 49 | 317 ± 52 | 758 ± 175 | 1676 ± 562 |
TA104 | 269 ± 40 | 332 ± 48 | 1973 ± 755 | 1196 ± 252 |
WP2uvrA | 29 ± 11 | 34 ± 11 | 273 ± 126 | 879 ± 177 |
WP2uvrA/pKM101 | 141 ± 43 | 198 ± 49 | 2080 ± 884 | 928 ± 250 |
results with 2 -aminoethanol
conc (µg/plate) | Number of revertants/plete (mean of 2 Experiments) | |||||||||||
| Base-substitution | Frame-shift | ||||||||||
Strain | TA100 | TA1535 | WP2uvrA | TA98 | TA1537 | TA1539 | ||||||
Metabolic acitvation | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 |
pos control | 942 | 462 | 258 | 172 | 274 | 1071 | 293 | 301 | 307 | 255 | 296 | 227 |
neg control | 205 | 197 | 17 | 11 | 23 | 22 | 23 | 31 | 11 | 9 | 9 | 16 |
50 | 203 | 215 | 26 | 12 | 29 | 26 | 19 | 38 | 9 | 22 | 11 | 13 |
100 | 198 | 208 | 30 | 10 | 15 | 28 | 21 | 32 | 9 | 13 | 9 | 15 |
200 | 199 | 218 | 17 | 12 | 23 | 23 | 16 | 32 | 10 | 14 | 9 | 20 |
500 | 205 | 220 | 22 | 12 | 19 | 21 | 19 | 30 | 6 | 20 | 7 | 15 |
1000 | 188 | 191 | 15 | 19 | 26 | 26 | 22 | 28 | 6 | 17 | 8 | 8 |
2000 | 72 | 148 | 12 | 15 | 15 | 29 | 14 | 21 | 4 | 8 | 6 | 8 |
5000 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Judgement | - | - | - | - | - | - | - | - | - | - | - | - |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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