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EC number: 233-332-1
CAS number: 10124-37-5
No reliable studies with calcium nitrate itself are available. CN-Nitcal
appeared to be not mutagenic in the Ames test and in a chromosome
aberration study. Potassium nitrate was negative in a Mouse Lymphoma
assay with and without metabolic activation. The read-across rationale
can be found in the document attached to the appropriate target records
and is fully incorporated in the CSR.
No biologically relevant effects of
CN-Nitcal on the number of polyploid cells and cells with
endoreduplicated chromosomes were observed both in the absence and
presence of S9-mix. Therefore it can be concluded that CN-Nitcal does
not disturb mitotic processes and cell cycle progression and does not
induce numerical chromosome aberrations.
No in vivo studies are required, as all in vitro studies showed no
No reliable studies with calcium nitrate are available. Nitric acid,
ammonium calcium salt is not mutagenic in Salmonella typhimurium strains
TA 1535, TA 1537, TA 98 and, TA 100 and E. Coli WP2 uvr A with and
without metabolic activation. Tests were performed according to OECD 471
and EU B.13/14 guidelines. No cytotoxicity was observed but test was
performed up to limit concentrations (5000 microg/plate). No chromosomal
aberrations were induced in human lymphocytes tested with and without
metabolic activation according to OECD 473 and B.10 guidelines. No
toxicity was observed up to and including the highest precipitating
tested dose in the absence and presence of S9, 3 hr treatment/24 hr
fixation. However toxicity was observed at dose levels of 3330 µg/ml and
above in the absence of S9 for the continuous treatment of 24 and 48 hr.
An MLA study has been performed with potassium nitrate (according to
OECD guideline 476 and EC guideline B.17), showing no effects on the
thymidine kinase locus in L5178Y mouse lymphoma cells. Test
concentrations were up to 0.01M, limit dose, with no toxicity.
Justification for selection of genetic toxicity endpoint
An Ames test and a CA test are available with the read-across substance
CN-Nitcal, all with a klimisch score 2, all relevant for showing no
adverse effect. A Mouse Lymphoma assay with the read-across substance
potassium nitrate is available with a klimisch score 2, relevant for
showing no adverse effect.
Based on all information available, calcium nitrate does not have to be
classified for genotoxicity according to the CLP Regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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