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EC number: 200-838-9 | CAS number: 75-09-2
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of cosmetic ingredients licensed by the European Communities
- Author:
- Gocke E, King M-T, Eckhardt K, Wild D
- Year:
- 1 981
- Bibliographic source:
- Mutat Res 90, 91-109
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dichloromethane
- EC Number:
- 200-838-9
- EC Name:
- Dichloromethane
- Cas Number:
- 75-09-2
- Molecular formula:
- CH2Cl2
- IUPAC Name:
- dichloromethane
- Details on test material:
- Dichloromethane was obtained from Merck Co., Darmstadt, Germany. The compound was checked for correct melting point and elementary analysis.
Constituent 1
Method
- Target gene:
- his
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from Aroclor-pretreated rats
- Test concentrations with justification for top dose:
- 125, 250, 500, and 750 μl in a 9 litre desiccator
- Vehicle / solvent:
- None
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- no
- Details on test system and experimental conditions:
- Because of the volatility of the compound the petri dishes were placed in airtight desiccators for 8 h. Substance was tested in Vogel-Bonner medium.
- Evaluation criteria:
- Not specified.
- Statistics:
- Not specified.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- The test substance was active in strains TA98 and TA100 with and without metabolic activation. In the figure below , increases up ca. 10-fold above control were noted in both strains. The increase was statistically significant (p<=0.01).
Any other information on results incl. tables
No data in table form were available, but a figure was included instead (see below).
Figure: results of the Ames test. Ordinate: His+ revertants per plate of the indicated strain(s), viz bullet = TA98 and triangle = TA100; abscissa: uL in 9 -L desiccator; closed symbol: in presence of S9 -mix, open symbol: without S9 -mix.
Applicant's summary and conclusion
- Conclusions:
- The test substance was active in strains TA98 and TA100 with and without metabolic activation.
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