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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
specific investigations: other studies
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: study with unclear GLP-status, peer-reviewed summary under auspices of WHO, restrictions in reporting

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
Methylene Chloride (2nd ed)
Author:
WHO
Year:
1996
Bibliographic source:
Environmental Health Criteria 164
Reference Type:
publication
Title:
Methylene chloride and chronic renal hypertension
Author:
Loyke HF
Year:
1973
Bibliographic source:
Arch Pathol 95 (2), 130-131

Materials and methods

Principles of method if other than guideline:
Not specified, but the review docuements (ECH 1996) considered the study results reliable/acceptable
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Dichloromethane
EC Number:
200-838-9
EC Name:
Dichloromethane
Cas Number:
75-09-2
Molecular formula:
CH2Cl2
IUPAC Name:
dichloromethane
Details on test material:
Not specified, but the review docuements (ECH 1996) considered the study results reliable/acceptable

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
not specified

Administration / exposure

Route of administration:
subcutaneous
Duration of treatment / exposure:
17 weeks
Frequency of treatment:
twice a week
Doses / concentrations
Remarks:
Doses / Concentrations:
2000 mg/kg bw
Basis:

Results and discussion

Details on results:
LOAEL = 2000 mg/kg bw (2 times/week for 17 weeks)

Any other information on results incl. tables

Reduction of the systolic blood pressure of hypertensive Sprague- Dawley rats was reported after subcutaneous exposure to 2000 mg/kg twice a week for 17 weeks. No effect was found in normotensive rats. Very slight histopathological changes in the liver and lungs of hypertensive rats were described.

Applicant's summary and conclusion