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Various studies were performed with Eisenia fetida (earthworm) and various higher plants. However, they were either not relevant for the endpoint or the orginal study could not be found and the available information was not sufficient.
Incubation of soil for 2 months with 1 -10 mg/kg (dry weight) dichloromethane reduced the activity of ß-glucosidase, ß-acetylglucosaminidase and proteinase during the first 28 days, with recovery after 2 months; no effect was observed at 0.1 mg/kg (Kanazawa and Filip 1986).