Registration Dossier

Toxicological information

Acute Toxicity: inhalation

Currently viewing:

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented publication, predates OECD guidelines and GLP, minor restrictions in design and reporting, but otherwise adequate for assessment

Data source

Reference
Reference Type:
publication
Title:
The toxicity and narcotic action of mono-chloromono- bromo-methane with special reference to inorganic and volatile bromide in blood, urine and brain.
Author:
Svirbely JL, Highman B, Alford WF, et al.
Year:
1947
Bibliographic source:
J Ind Hyg Toxicol 29 (6), 382-389

Materials and methods

Principles of method if other than guideline:
Mice were exposed for 7 h by whole-body exposure to dichloromethane vapour in a 15-L bell jar.
GLP compliance:
no
Test type:
other: 7-hour whole body exposure
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): di-chloro-methane (methylene chloride)

Test animals

Species:
mouse
Strain:
Swiss Webster
Sex:
not specified
Details on test animals and environmental conditions:
White Swicce mice of approx. 20 g body weight were used

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
Mice were exposed in a bell jar of approximately 15 liters capacity.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
7 h
Concentrations:
53.5, 45.6, 47.7 and 39.9 mg/l (determined) (154000, 13126, 13730 and 11485 ppm)
No. of animals per sex per dose:
20/concentration
Control animals:
no
Details on study design:
The observation period was one day.

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
49 000 mg/m³ air
Based on:
test mat.
Exp. duration:
7 h
Clinical signs:
other: The animals became restless, developed muscular twitchings, uncoordinated movements, labored respiration, and narcosis.

Any other information on results incl. tables

Table 1. The acute toxicity of vapours of dichloromethane for mice.

Concentration in air

 

 

 

 

 

 

Number of fatalities following exposure

 

 

Calculated

 

Determined

 

Hours

 

mg/liter

ppm

mg/liter

ppm

8

24

 58.70

16.897 

53.5 

15.400 

18/20 

 

 55.55

15.990 

45.6 

13.126 

4/20 

5/20 

 52.94

15.239 

47.7 

13.730 

2/20 

 

 44.45

12.795 

39.9 

11.485 

0/20 

 

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Because the 4-h LC50 value (calculated from a 7-h value) was 86 mg/L, and no delayed mortality was expected, no classification is needed for acute inhalation toxicity.