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EC number: 250-954-9 | CAS number: 32210-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 18.03.1997 to 16.04.1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was performed according to EU Method C.4-C with GLP statement. All validity criteria were fulfilled and no deviations from the protocol were observed.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): From Municipal activated sludge plant (Marl, East).
- Concentration of sludge: content of suspended solids = 29.7 mg/L. Dry matter content of the inoculum used = about 4.4 g/L.
- Initial cell/biomass concentration: 122.10^4 CFU (colony forming units)/mL - Duration of test (contact time):
- 29 d
- Initial conc.:
- ca. 20.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 15 mg/L
- Based on:
- other: C
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
2 days before testing, 40 L of mineral medium were made and ventilated overnight. For the test, inoculation with inoculum up to 3L with mineral medium.
- Composition of medium: no data
- Additional substrate: no data
- Solubilising agent (type and concentration if used): None
- Test temperature: 20.4 to 23.6°C (mean of 22.1°C)
- pH: 7.5 to 7.6
- pH adjusted: no
- Aeration of dilution water: yes
TEST SYSTEM
- Culturing apparatus: 5 L Glass vessel
- Number of culture flasks/concentration: 1 concentration / 4 vessels
- Method used to create aerobic conditions: no data
- Measuring equipment: TOC analysis
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (2 vessels)
- Abiotic sterile control: no
- Positive control: yes (1 vessel) - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- None
- Test performance:
- None
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 75
- Sampling time:
- 29 d
- Remarks on result:
- other: 10 day window fulfilled
- Details on results:
- See tables in "Any other information on results incl. tables".
- Results with reference substance:
- 88% biodegradation after 14 and 29 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradation of the test substance was 75% after 28 days and the 10 days window was fulfilled. Based on these results, the test substance is considered to be readily biodegradable.
- Executive summary:
This study was performed according to modified Sturm test as specified by EC Directive 92/69/EEC C.4 -C with GLP statement, to assess the biodegradation of the test substance for 29 days under aerobic conditions.
The test substance was applied at the rate of c.a 20.7 mg/L of mineral water (without solvent) with activated sludge (4 vessels). To validate the results, two inoculum control vessels (without test substance) and one positive control vessel (reference substance: sodium benzoate with inoculum) were performed. The CO2 evolution was recorded during 29 days (0, 1, 2, 3, 4, 5, 9, 14, 15, 20, 24, 28 and 29 days) by TOC-analysis. The temperature during the test was comprised between 20.4 and 23.6°C with a mean of 22.1°C. The pH was comprised between 7.5 and 7.6.
All validity criteria were fulfilled. The biodegradation of the reference substances was 88% after 14 and 29 days. The biodegradation of the test substance was 75% after 28 days (also after 29 days) and the test substance was degraded by > 60% within 10 days after the time at which the biodegradation has reached 10%.
Based on these results, the test substance is considered to be readily biodegradable.
Reference
Table 5.2.1/1: Raw data
mg TOC/L 0.05 N NaOH |
||||||||
Days (d) |
Control |
Mean Control |
Reference substance |
Test substance |
||||
20.6 mg/L |
20.7 mg/L |
20.8 mg/L |
20.6 mg/L |
|||||
30min. 1 2 3 4 5 9 14 15 20 24 28 29 |
1.61 3.02 4.00 5.48 7.21 8.24 12.10 15.69 16.35 18.81 20.01 20.70 20.91 |
1.35 2.79 3.63 4.85 6.60 7.56 11.10 14.58 15.27 18.27 19.68 20.37 20.85 |
1.48 2.91 3.82 5.17 6.91 7.90 11.60 15.14 15.81 18.54 19.85 20.54 20.88 |
1.29 12.82 26.07 33.85 37.95 40.95 47.70 53.62 54.67 57.61 58.38 59.01 59.57 |
1.09 3.28 5.55 8.38 10.57 12.15 17.10 40.20 43.35 50.75 51.45 54.04 53.97 |
1.11 3.18 5.50 7.56 10.48 12.06 17.97 44.30 46.70 52.75 54.04 55.86 56.35 |
1.20 3.54 5.50 6.69 10.41 13.11 18.63 40.90 44.00 51.55 52.36 54.18 54.53 |
1.04 3.65 5.69 7.91 10.70 13.09 18.60 40.75 44.05 51.05 51.66 54.18 54.88 |
Table 5.2.1/2: Biodegradation of the test and reference substances
Days (d) |
Test substance - % biodegradation |
Mean % biodegradation of test substance |
Reference substance - % biodegradation |
|||
20.6 mg/L |
20.7 mg/L |
20.8 mg/L |
20.6 mg/L |
|||
30min. 1 2 3 4 5 9 14 15 20 24 28 29 |
-1 1 4 7 8 9 12 56 61 72 70 74 73 |
-1 1 4 5 8 9 14 65 69 76 76 78 79 |
-1 1 4 3 8 12 16 57 62 73 72 74 74 |
-1 2 4 6 8 12 16 57 63 72 71 75 75 |
-1 1 4 5 8 11 15 59 64 73 72 75 75 |
0 23 51 65 71 75 82 88 89 89 88 88 88 |
Description of key information
EU Method C.4-C, GLP, key study, validity 1:
75% biodegradation after 28 days within the 10 day window.
Readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
One valid key study is available to assess the biodegradation potential of the registered substance. This study was performed according to modified Sturm test as specified by EC Directive 92/69/EEC C.4 -C with GLP statement, to assess the readily biodegradation of the registered substance for 29 days under aerobic conditions. The substance attained 75% biodegradation after 28 and 29 days of incubation within the 10 day window.
Therefore, the substance can be considered to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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