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EC number: 250-954-9 | CAS number: 32210-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- December 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Old study (pre-OECD and pre-GLP) but comparable to OECD test guideline No. 402 with deviations: only 3 rabbits per sex were used.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- only 3 animals/sex; environmental conditions not reported
- Principles of method if other than guideline:
- Protocol #DLD (11/7/78) supplied by IFF and dated 12 October 1978
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- trans-4-tert-butylcyclohexyl acetate
- EC Number:
- 217-598-6
- EC Name:
- trans-4-tert-butylcyclohexyl acetate
- Cas Number:
- 1900-69-2
- Molecular formula:
- C12H22O2
- IUPAC Name:
- trans-4-tert-butylcyclohexyl acetate
- Reference substance name:
- cis-4-tert-butylcyclohexyl acetate
- EC Number:
- 233-881-7
- EC Name:
- cis-4-tert-butylcyclohexyl acetate
- Cas Number:
- 10411-92-4
- Molecular formula:
- C12H22O2
- IUPAC Name:
- cis-4-tert-butylcyclohexyl acetate
- Test material form:
- liquid
- Details on test material:
- - Description: clear liquid
Constituent 1
Constituent 2
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: C.S.E. colony located in Brancheville, N.J.
- Age at study initiation: young adult
- Weight at study initiation: 2.45-3.50 kg
- Housing: singly
- Diet (e.g. ad libitum): Purina Rabbit Chow
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 7 days
ENVIRONMENTAL CONDITIONS
Standard laboratory conditions
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: shaved skin on the back
- % coverage: approximately 20 %
- Type of wrap if used: plastic sleeves ('Baggies')
REMOVAL OF TEST SUBSTANCE
- Washing (if done): disposable napkin moistened with saline
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5 mL/kg bw
Rabbits were placed in restrainers for the 24 hour period following application. - Duration of exposure:
- 24 hours
- Doses:
- 5 mL/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations (signs of toxicity and death): twice daily (once daily on Saturday and Sunday)
- Frequency of observations and weighing: on the day of dosing and before necropsy
- Necropsy of survivors performed: yes - Statistics:
- none
Results and discussion
- Preliminary study:
- not applicable
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 mL/kg bw
- Based on:
- test mat.
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- >= 5 mL/kg bw
- Based on:
- test mat.
- Mortality:
- No animal died during the study
- Clinical signs:
- other: Slight to moderate erythema was observed during the first 24 hours after application. All rabbits exhibited a slight scaliness over the treated area, which was first observed on Day 8 and persisted to the end of the study.
- Gross pathology:
- Agonal subcapsular haemorrhage observed in the right or left kidney in 2 rabbits. Pale liver in 1 rabbit.
- Other findings:
- None
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Dermal LD50Combined > 5 mL/kg bw (> 4680 mg/kg bw)
Under the test conditions, the test material is not classified according to the Regulation EC No. 1272/2008 (CLP) and to the GHS. - Executive summary:
In a limit acute dermal toxicity study performed before but similarly to the OECD guideline No. 402, groups of young adult albino New Zealand rabbits (3/sex) were occlusively exposed to undiluted test material for 24 hours at dose of 5 mL/kg bw (ca. 4680mg/kg bw; density = 0.936). The animals were observed for mortality, clinical signs including dermal reactions and body weight for 14 days and then necropsied for macroscopic observations.
No mortality occurred during the study and no clinical signs were observed. There was no adverse effect on bodyweight gain. Slight to moderate erythema was observed during the first 24 hours after application. All rabbits exhibited a slight scaliness over the treated area, which was first observed on Day 8 and persisted to the end of the study.
Dermal LD50Combined > 5 mL/kg bw (> 4680 mg/kg bw)
Under the test conditions, the test material is not classified according to the Regulation EC No. 1272/2008 (CLP) and to the GHS.
This study is considered as acceptable and satisfies the requirement for acute dermal toxicity endpoint.
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