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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented study performed according OECD 202 and GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulphonyl chloride
EC Number:
202-636-6
EC Name:
Benzenesulphonyl chloride
Cas Number:
98-09-9
Molecular formula:
C6H5ClO2S
IUPAC Name:
benzenesulfonyl chloride
Details on test material:
- Name of test material (as cited in study report): Benzenesulphonyl chloride (Provichem 0203)
- Analytical purity: 99.95%
- Production date: 04/11/2009
- Lot/batch No.: 200911040020
-Shelf-life: 1 year
-Date of receipt: 05/02/2010
- Storage condition of test material: ambient room temperature 20-30°C
- Other:

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples from the exposure media were collected from control, 5 and 80 mg/L concentrations at 0 and 48 hours and sent to Analytical Chemistry laboratory for verification of test concentrations.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: no data
- Eluate: no data
- Differential loading: no data
- Controls: no data
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Daphnia magna culture, IIBAT. Primary culture supplied by M/s Marinco Bioassay lab, U.S.A
- Age at study initiation (mean and range, SD):Daphnia magna less than 24 hours old were removed from the culture and used for the experiment.
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data
- Peripheral shell growth removed prior to test initiation: no data
- Feeding during test: no
- Brood daphnids from the culture was maintained in M4 medium in the test room at the test temperature prior to start of the experiment.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
no data

Test conditions

Hardness:
162 mg/L as CaCO3
Test temperature:
18.0-19.4 °C
pH:
6.46-6.97 (except pH 4.63 at start of test with 80 mg/L)
Dissolved oxygen:
7.90-8.48 mg/L
Nominal and measured concentrations:
Nominal: control; 5; 10; 20; 40 and 80 mg/L
Measured concentrations were 5 and 80 mg/L, beginning and end of test had a recovery of more than 96%, so nominal values can be used
Details on test conditions:
TEST SYSTEM
- Test vessel: test beaker (100 mL)
- Exposure medium: M4 medium (50 mL)
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- Renewal rate of test solution (frequency/flow rate): static
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium ( M4 medium nutrients are listed in Table 1)


OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: no data
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Range finding study: 0.01; 0.1; 1.0; 10 and 100 mg/L
- Test concentrations: 5; 10; 20; 40; 80 mg/L
Reference substance (positive control):
yes
Remarks:
postassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
66.93 mg/L
Nominal / measured:
nominal
Basis for effect:
other: immobilisation
Remarks on result:
other: 51.54-82.32 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
41.61 mg/L
Nominal / measured:
nominal
Basis for effect:
other: immobilisation
Remarks on result:
other: 33.31-49.91 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Details on results:
- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no data
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: no data
- EC50/LC50: 0.70 mg/L
- Other:
Reported statistics and error estimates:
EC50 (with 95% confidence limits), LOEC and NOEC (by Fisher’s exact test) was calculated using a software program, TOXSTAT 3.5 Version (WEST, Inc. and Dave Gulley, Univ. of Wyoming; 2003, Central Avenue, Cheyenne, WY82001, USA) and NCSS (2000).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based upon the above experiment, the 24 and 48 hour EC50 (with 95% Confidence limits), NOEC and LOEC of Benzenesulphonyl chloride (Provichem 0203) for Daphnia magna was determined as follows:

EC50 - 24 hours : 66.93 mg/L (95% Confidence limits: 51.54 to 82.32mg/L)
EC50 - 48 hours : 41.61 mg/L (95% Confidence limits: 33.31 to 49.91 mg/L)
NOEC (48 hours) : 10 mg/L
LOEC (48 hours) : 20 mg/L