Benzenesulphonyl chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study performed according OECD 202 and GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulphonyl chloride

EC Number:

202-636-6

EC Name:

Benzenesulphonyl chloride

Cas Number:

98-09-9

Molecular formula:

C6H5ClO2S

IUPAC Name:

benzenesulfonyl chloride

Details on test material:

- Name of test material (as cited in study report): Benzenesulphonyl chloride (Provichem 0203)
- Analytical purity: 99.95%
- Production date: 04/11/2009
- Lot/batch No.: 200911040020
-Shelf-life: 1 year
-Date of receipt: 05/02/2010
- Storage condition of test material: ambient room temperature 20-30°C
- Other:

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples from the exposure media were collected from control, 5 and 80 mg/L concentrations at 0 and 48 hours and sent to Analytical Chemistry laboratory for verification of test concentrations.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: no data
- Eluate: no data
- Differential loading: no data
- Controls: no data
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna culture, IIBAT. Primary culture supplied by M/s Marinco Bioassay lab, U.S.A
- Age at study initiation (mean and range, SD):Daphnia magna less than 24 hours old were removed from the culture and used for the experiment.
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Valve height at study initiation, for shell deposition study (mean and range, SD): no data
- Peripheral shell growth removed prior to test initiation: no data
- Feeding during test: no
- Brood daphnids from the culture was maintained in M4 medium in the test room at the test temperature prior to start of the experiment.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

no data

Test conditions

Hardness:

162 mg/L as CaCO3

Test temperature:

18.0-19.4 °C

pH:

6.46-6.97 (except pH 4.63 at start of test with 80 mg/L)

Dissolved oxygen:

7.90-8.48 mg/L

Nominal and measured concentrations:

Nominal: control; 5; 10; 20; 40 and 80 mg/L
Measured concentrations were 5 and 80 mg/L, beginning and end of test had a recovery of more than 96%, so nominal values can be used

Details on test conditions:

TEST SYSTEM
- Test vessel: test beaker (100 mL)
- Exposure medium: M4 medium (50 mL)
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- Renewal rate of test solution (frequency/flow rate): static
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium ( M4 medium nutrients are listed in Table 1)


OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: no data
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Range finding study: 0.01; 0.1; 1.0; 10 and 100 mg/L
- Test concentrations: 5; 10; 20; 40; 80 mg/L

Reference substance (positive control):

yes

Remarks:

postassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

66.93 mg/L

Nominal / measured:

nominal

Basis for effect:

other: immobilisation

Remarks on result:

other: 51.54-82.32 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

41.61 mg/L

Nominal / measured:

nominal

Basis for effect:

other: immobilisation

Remarks on result:

other: 33.31-49.91 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Details on results:

- Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: no data

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: no data
- EC50/LC50: 0.70 mg/L
- Other:

Reported statistics and error estimates:

EC50 (with 95% confidence limits), LOEC and NOEC (by Fisher’s exact test) was calculated using a software program, TOXSTAT 3.5 Version (WEST, Inc. and Dave Gulley, Univ. of Wyoming; 2003, Central Avenue, Cheyenne, WY82001, USA) and NCSS (2000).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based upon the above experiment, the 24 and 48 hour EC50 (with 95% Confidence limits), NOEC and LOEC of Benzenesulphonyl chloride (Provichem 0203) for Daphnia magna was determined as follows:

EC50 - 24 hours : 66.93 mg/L (95% Confidence limits: 51.54 to 82.32mg/L)
EC50 - 48 hours : 41.61 mg/L (95% Confidence limits: 33.31 to 49.91 mg/L)
NOEC (48 hours) : 10 mg/L
LOEC (48 hours) : 20 mg/L