Benzenesulphonyl chloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010-07-14 to 2010-07-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study performed according OECD 201 and GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulphonyl chloride

EC Number:

202-636-6

EC Name:

Benzenesulphonyl chloride

Cas Number:

98-09-9

Molecular formula:

C6H5ClO2S

IUPAC Name:

benzenesulfonyl chloride

Details on test material:

- Name of test material (as cited in study report): Benzenesulphonyl chloride (Provichem 0203)
- Analytical purity: 99.95%
- Production date: 04/11/2009
- Lot/batch No.: 200911040020
-Shelf-life: 1 year
-Date of receipt: 05/02/2010
- Storage: between 20 and 30°C

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control; 100 mg/L
- Sampling method: HPLC
- Sample storage conditions before analysis: no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg/L concentration of Benzenesulphonyl chloride (Provichem 0203) was prepared by dissolving 71.10 mg of Benzenesulphonyl chloride (Provichem 0203) in 711 mL of OECD TG 201 medium. 100 mL of test item was tranferred into sterile 250 mL Erlenmeyer flasks. The control and treated flasks were inoculated with 90 microL of P. subcapitata from the pre-culture to obtain initial cell concentrations of about 1x10000 cells per mL.
- Differential loading:no data
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no vehicle
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no indication of precipitation

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: no data
- Strain: SAG 61.81
- Source (laboratory, culture collection): primary culture obtained from University of Gottingen, Germany
- Age of inoculum (at test initiation): no data
- Method of cultivation: no data

ACCLIMATION
- Acclimation period: pre-culture of P. subcapitata was done three days before initiation of the study
- Culturing media and conditions (same as test or not): same; 6780-6842 lux light intensity at 22.6 to 23.3 °C for three days
- Any deformed or abnormal cells observed: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

no data

Test conditions

Hardness:

no data

Test temperature:

22.6 - 23.3°C

pH:

test inititation: 8.09-8.13
test termination: 7.74-7.88

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

Nominal: control; 100 mg/L
Measured: The mean recovery values were 99.48% at test initiation and 98.27% at test termination.

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL flask Erlenmeyer
- Initial cells density: 10000 cells per mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


GROWTH MEDIUM
- Standard medium used: yes


TEST MEDIUM / WATER PARAMETERS
- according OECD 201

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: to 8.1
- Photoperiod: no data
- Light intensity and quality: 6780-6842 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]: visually counted using an Improved Neubaur's Haemocytometer under illumination of the microscope after 24, 48 and 72 h after inoculation


TEST CONCENTRATIONS
- Range finding study: 0.1; 1.0; 10; 50; and 100 mg/L
- Test concentrations:100 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

postassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 98.88 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control (for algal test): initial cell count 10000 cells/mL and avarage final cell count at 72h was 180 x 10000 cells/mL.
- Observation of abnormalities (for algal test): no data
- Unusual cell shape: no data
- Colour differences: no data
- Flocculation: no data
- Adherence to test vessels: no data
- Aggregation of algal cells: no data
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 0.35 mg/L (95% confidence limits: 0.34 to 0.36 mg/L)
0.94 mg/L (95% confidence limits: 0.91 to 0.97 mg/L)

- Other:

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the study results, EyC50 (0-72 h) and ErC50 (0-72 h) of Benzenesulphonyl chloride (Provichem 0203) was determined as >100 mg/L. The NOEC and LOEC of Benzenesulphonyl chloride (Provichem 0203) were calculated using 2 sample t-test (TOXSTAT 3.5 VERSION) and determined as 100 mg/L.