Benzenesulphonyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / micronucleus study

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according OECD guideline 487

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 487

GLP compliance:

yes

Type of assay:

in vitro mammalian cell micronucleus test

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulphonyl chloride

EC Number:

202-636-6

EC Name:

Benzenesulphonyl chloride

Cas Number:

98-09-9

Molecular formula:

C6H5ClO2S

IUPAC Name:

benzenesulfonyl chloride

Details on test material:

- Name of test material (as cited in study report): Benzène sulfochlorure
- Physical state: colourless liquid
- Analytical purity: 99.73%
- Lot/batch No.: S24711-466
- Storage condition of test material: room temperature

Method

Species / strain

Species / strain / cell type:

mouse lymphoma L5178Y cells

Details on mammalian cell type (if applicable):

- Source: ATCC
- Properly maintained: stored at -180°C in liquid nitrogen
- Periodically checked for Mycoplasma contamination: yes

Metabolic activation:

with and without

Metabolic activation system:

S9 from liver and S9 from kidney

Test concentrations with justification for top dose:

Test 1: S9- (3h/+21h) : (0.078) - 0.156 - 0.312 - 0.625 - 1.25 - (2.5) - (5) - (10) µg/ml
Test 2: S9- (24h) : (0.078) - (0.156) - (0.312) - 0.625 - 1.25 - 2.5 - 5 - (10) µg/ml
Test 3: S9+/5% S9 liver (3h/+21h) : (0.312) - 0.625 - 1.25 - 2.5 - 5 - (10) - (20) - (40) µg/ml
Test 4: S9+/5% S9 kidney (3h/+21h) : (0.312) - 0.625 - 1.25 - 2.5 - 5 - (10) - (20) - (40) µg/ml
Values between brackets have not been retained for evaluation

Vehicle / solvent:

-ethanol absolu (MERCK, lot K365 899 983 638)

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: Test 1: Mitomycine C 0.2 µg/ml and Taxol 25 µg/ml. Test 2: Mitomycine C 0.1 µg/ml and Griseéofulvine 12.5 µg/ml. Test 3: Cyclophosphamide 6 µg/ml. Test 4: Streptozotocine 500 µg/ml and Streptozotocine 250 µg/ml.

Details on test system and experimental conditions:

METHOD OF APPLICATION: added in medium

DURATION
- Exposure duration: 24h

NUMBER OF REPLICATIONS: 2

NUMBER OF CELLS EVALUATED: 1000 per test

DETERMINATION OF CYTOTOXICITY
- Method: % of relative survival/control

Results and discussion

Test results

Species / strain:

mouse lymphoma L5178Y cells

Metabolic activation:

with and without

Genotoxicity:

positive

Remarks:

Genotoxic with metablic activation from kidney at concentration of 2.5µg/ml. Not genotoxic without metabolic activation and with metabolic activation from liver.

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

Moderate to strong cytotoxic at concentrations of 2.44 µg/ml to 625 µg/ml without metabolic activation and moderate to strong cytotoxic at concentrations of 4.88 or 9.77 µg/ml to 625 µg/ml with metabolic activation from kidney and liver respectively

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: strain/cell type: mouse lymphoma L51784 cells/ATCC

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive with metabolic activation kidney