Triphenylphosphine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: test procedure in accordance with generally accepted standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: The acute dermal toxicity to rabbits was evaluated according to a BASF-internal standard method by a single application of an 50% alcoholic suspension to the shaven dorsal skin of 3 male and 3 female animals, equivalent to a dose of 4000 mg/kg test material. During 24 hour exposure period, the exposed skin area of 355 cm2 was covered with parchment paper and overlying bandages and the animals were restrained. After application, the animals were observed for 14 days for mortality and syptoms of acute intoxication or local irritation. Animals were evaluated for gross pathological changes. The mean lethal dose was determined

GLP compliance:

no

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine

EC Number:

210-036-0

EC Name:

Triphenylphosphine

Cas Number:

603-35-0

Molecular formula:

C18H15P

IUPAC Name:

triphenylphosphine

Test material form:

solid: crystalline

Specific details on test material used for the study:

- Purity: 99%

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a dissolved solid, stock liquid or gel: 50% alcoholic suspension

FORM AS APPLIED IN THE TEST (if different from that of starting material): suspension

Test animals

Species:

rabbit

Strain:

Vienna White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: 2/6 bred by the medical-biological research laboratory of BASF, 4/6 purchased.
- Weight at study initiation: average 3,24 kg.
- Diet (e.g. ad libitum): yes.
- Water (e.g. ad libitum): yes.

IN-LIFE DATES: From: 14.10.1971 To: 28.10.1971.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

ethanol

Details on dermal exposure:

TEST SITE
- Area of exposure: 355 cm2.
- Type of wrap if used: the application area was covered with parchment paper and overlying bandages.

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): approx. 10 mL.
- Concentration (if solution): 50 %.

Duration of exposure:

24 hours

Doses:

4000 mg/kg

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days.
- Frequency of observations: at least after 1h, 24h, 48h, 7d and 14d.
- Necropsy of survivors performed: yes.
- Other examinations performed: clinical signs, signs of local irritation.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

approximate LD50

Effect level:

> 4 000 mg/kg bw

Remarks on result:

other: no mortalities

Mortality:

No mortalities occured.

Clinical signs:

other: No signs of acute intoxication and local irritation were observed.

Gross pathology:

Necropsy revealed no macroscopic and/or organ pathological findings related to TS exposure.

Any other information on results incl. tables

Table 1: Mortality

Dose [mg/kg]	Concentration in alcohol [%]	Number of animals	Dead animals within
			1 hour	24 hours	48 hours	7 days	14 days
4000	50	3 male 3 female	0 / 3 0 / 3	0 / 3 0 / 3	0 / 3 0 / 3	0 / 3 0 / 3	0 / 3 0 / 3

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

LD50 > 4000 mg/kg

Executive summary:

The acute dermal toxicity of the test substance to rabbits was assessed according to a BASF-internal standard method. Therefore, the test substance was applied as an 50% alcoholic suspension to the shaven backs of 6 rabbits for 24 hours, equivalent to a dose of 4000 mg/kg test substance . During a 14-day observation period, mortality, signs of acute intoxication or local irritation were recorded. Pathological changes were evaluated in all animals. No mortalities occured. No signs of acute intoxication and local irritation were observed. Necropsy revealed no macroscopic and/or organ pathological findings related to test substance exposure. Therefore the dermal LD50 was determined to be > 4000 mg/kg.