Triphenylphosphine

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Study period:

October 1950 and September 1951

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: scientifically acceptable

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1952

Report date:

1952

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: BASF-internal standard method. The acute toxicity to rats after a single subcutaneous administration of a 20% oily suspension, equivalent to doses of 1000, 3000, 4000, 5000 and 6000 mg/kg test material, was evaluated. After application, the animals were observed 8 days for mortality and syptoms of acute intoxication. Dead animals, time to death and symptoms of acute intoxication were recorded. The mean lethal dose was determined.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine

EC Number:

210-036-0

EC Name:

Triphenylphosphine

Cas Number:

603-35-0

Molecular formula:

C18H15P

IUPAC Name:

triphenylphosphine

Test material form:

solid: crystalline

Specific details on test material used for the study:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Final dilution of a suspended solid: 20 %

FORM AS APPLIED IN THE TEST (if different from that of starting material): oily suspension

Test animals

Species:

mouse

Strain:

other: albino

Sex:

not specified

Administration / exposure

Route of administration:

subcutaneous

Vehicle:

other: 20% TS in olive oil

Doses:

1000, 3000, 4000, 5000, 6000 mg/kg bw

No. of animals per sex per dose:

1000 mg/kg bw: 5 animals
3000 mg/kg bw: 8 animals
4000 mg/kg bw: 8 animals
5000 mg/kg bw: 8 animals
6000 mg/kg bw: 3 animals

Control animals:

no

Details on study design:

- Duration of observation period following administration: 8 days.
- Frequency of observations: at least daily.
- Necropsy of survivors performed: no.
- Other examinations performed: clinical signs.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LD50

Effect level:

ca. 3 000 mg/kg bw

Remarks on result:

other: Mortality occured.

Mortality:

Mortality of 5/8, 7/8, 7/8, 3/3 animals was observed in dose groups of 3000, 4000, 5000 and 6000 mg/kg bw, respectively. Death occured within 1-3 days after application. At 1000 mg/kg bw no animal died.

Clinical signs:

Animals that died showed tonic-clonic seizures, accelerated breathing and impaired balance. No clinical findings in all surviving animals.

Any other information on results incl. tables

Table 1. Mortality

Dose [mg/kg]	Number of animals	Dead animals	Time to death [days]
6000	3	3/3	1
5000	8	7/8	1-2
4000	8	7/8	1-3
2000	8	5/8	1
1000	5	0/5	-

Applicant's summary and conclusion

Executive summary:

Single doses of 1000 to 6000 mg/kg bw were administered subcutanoeously to 3 (high dose), 5 (low dose) or 8 (mid dose groups) mice per group as 20-% suspension in olive oil. Mortality of 5/8, 7/8, 7/8, 3/3 animals was observed in dose groups of 3000, 4000, 5000 and 6000 mg/kg bw, respectively. Death occurred within 1-3 days after application. At 1000 mg/kg bw no animal died during an observation period of 8 days. Animals that died showed tonic-clonic seizures, accelerated breathing and impaired balance, whereas surviving animals appeared normal. An LD50 of 3000 mg/kg was determined.