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Diss Factsheets

Toxicological information

Repeated dose toxicity: other routes

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Administrative data

Endpoint:
repeated dose toxicity: other route
Type of information:
experimental study
Adequacy of study:
other information
Study period:
18.03. - 02.04.1952
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: screening test: discrepancy between documented test parameters and standard methods; only 2 animals were used.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1952
Report date:
1952

Materials and methods

Principles of method if other than guideline:
- Procedure: A suspension of 10- or 20-% test substance in olive oil equivalent to a dose of 300 mg/kg was applied daily (5 injection per week, max. 1 injection per day) intramuscular to one rabbit. The animal was observed for clinical signs of intoxication and mortality. the deceased animal was inspected for macroscopic and microscopic pathological changes.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Triphenylphosphine
EC Number:
210-036-0
EC Name:
Triphenylphosphine
Cas Number:
603-35-0
Molecular formula:
C18H15P
IUPAC Name:
triphenylphosphine
Test material form:
solid: crystalline

Test animals

Species:
rabbit

Administration / exposure

Route of administration:
intramuscular
Vehicle:
olive oil
Duration of treatment / exposure:
13 days
Frequency of treatment:
1x/d, max. 5x/wk
Doses / concentrations
Dose / conc.:
300 mg/kg bw/day (nominal)
No. of animals per sex per dose:
2
Control animals:
no
Details on study design:
Post-exposure period: 2 days

Results and discussion

Results of examinations

Clinical signs:
effects observed, treatment-related
Description (incidence and severity):
Signs of intoxication were observed from day 10 after the start of the study.
The animal showed ataxia and paralysis of the hind limbs from day 10 on and apathy and atony of the whole body at day13.
Before death, lateral positioning and labored breathing was observed.
Mortality:
mortality observed, treatment-related
Description (incidence):
A dose of 300 mg/kg was lethal after 15 days (9 injections).
Gross pathological findings:
no effects observed
Description (incidence and severity):
Macroscopic pathology revealed no characteristic substance-related findings: cachexia, cardiac flaccidity, collapsed/condensed lungs in one case, no significant lesions of the CNS.
Neuropathological findings:
no effects observed
Description (incidence and severity):
Histopathology including CNS revealed no relevant effects.

Applicant's summary and conclusion

Executive summary:

A suspension of 10- or 20-% test substance in olive oil equivalent to a dose of 300 mg/kg was applied daily (5 injection per week, max. 1 injection per day) intramuscular to one rabbit. The dosing was paused from day 14 on due to severe clinical symptoms.

A dose of 300 mg/kg was lethal after 15 days (9 injections).

The animal showed ataxia and paralysis of the hind limbs from day 10 on and apathy and atony of the whole body at day13.

Before death, lateral positioning and labored breathing was observed.

Macroscopic and microscopic pathology revealed no characteristic substance-related findings: cachexia, cardiac flaccidity, collapsed/condensed lungs in one case, no significant lesions of the CNS.