Triphenylphosphine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable test method without detailed documentation; reliable experimental data

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1975

Materials and methods

Principles of method if other than guideline:

- Principle of test: 6 male rats were whole body exposed to an aerosol of the test substance for 4 hours. Therefore, the test stubstance was molten to liquify, nebulized under a stream of dry nitrogen and diluted with 20 % oxygen. The animals were observed 7 days for mortality and syptoms of acute intoxication. Animals were examiened for gross pathological and histopathological changes.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine

EC Number:

210-036-0

EC Name:

Triphenylphosphine

Cas Number:

603-35-0

Molecular formula:

C18H15P

IUPAC Name:

triphenylphosphine

Test material form:

solid: crystalline

Test animals

Species:

rat

Strain:

other: Chr-CD (Charles River-CD)

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charales River.
- Age at study initiation: adult.
- Weight at study initiation: 250-275 grams.

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

other: nitrogen stream diluted with 20 % oxygen

Remark on MMAD/GSD:

particel size distribution not specified

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: boroilicate glass bell jars.
- Exposure chamber volume: 18 liter.
- System of generating particulates/aerosols: The test substance was nmelted in a glass flask and the molten material maintained at 90-115°C under dry, prepurified nitrogen . It was nebulized with a stainless steel nebulizer. Immediately prior
to entering the exposure chamber, the nitrogen-diluted stream of each material under study was again diluted with cylinder oxygen to an oxygen content of 20% (v/v) .

TEST ATMOSPHERE
- Brief description of analytical method used: Atmospheric samples were collected in an evacuated gas-sampling bulb of known volume. The sample was quantitatively transferred from the bulb with absolute alcohol and the washings diluted to a known volume. To calculate test substance concentrations, the O.D. of this solution was determined at 260 nm. The lower limit of quantitative detection by this method was 1.9 moles (500 ug) of test substance. The standard curve was linear for the ranges of concentrations used. Chamber atmospheres were analyzed at least hourly.

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

not specified

No. of animals per sex per dose:

6

Control animals:

no

Details on study design:

- Duration of observation period following administration: not specified
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology, histopathology

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

12.5 mg/L air

95% CL:

>= 8.6 - <= 18.2

Exp. duration:

4 h

Remarks on result:

other: 32.6 - 69.6 μmol/l

Mortality:

No individual data on mortality available.

Clinical signs:

other: Clinical signs were typical of respiratory irritation: red ears, salivation, lacrimation, facepawing and dyspnea.

Gross pathology:

No macroscopic or histopathological findings.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

LC50 = 12.5 mg/L

Executive summary:

Six male rats were whole body exposed to an aerosol of the test substance for 4 hours. Therefore, the test stubstance was molten to liquify, nebulized under a stream of dry nitrogen and diluted with 20 % oxygen prior to entering the exposure chamber. The animals were observed 7 days for mortality and syptoms of acute intoxication. Animals were examiened for gross pathological and histopathological changes. The animals showed clinical signs typical of respiratory irritation: red ears, salivation, lacrimation, facepawing and dyspnea. No macroscopic or histopathological effects were observed. No individual data on mortality were reported, however, a LC50 of 12.5 mg/L was stated.