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EC number: 700-342-7 | CAS number: 1163775-81-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-04-01 - 2015-11-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- N/A
- Analytical monitoring:
- yes
- Details on sampling:
- The samples were analyzed directly without storage.
- Vehicle:
- no
- Details on test solutions:
- Elendt M4 medium was prepared by analytical reagent and deionized water and stored at 2 to 8 °C. The concentration of TOC in the medium (i.e. before addition of the algae) was measured regularly every year and it was 1.74 mg/L. The test medium was aerated for more than 30 minutes prior to the start of the test and placed in testing temperature at least 12h before the test. For the medium at the beginning and during the test, the dissolved oxygen concentration was 8.81-9.44mg/L; the pH was 7.79-8.35; the hardness was 165-175 mg/L(as CaCO3).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus
- Source: supplied by MicroBioTests lnc.and not first brood progeny were used at the start of the test.
- Age at study initiation: not older than 24h
- Method of breeding: Adult Daphnia magna were bred in the laboratory of APM in the Elendt M4 medium. The culture conditions were similar to test conditions.
- Feeding during test: Cultures of adult Daphnia magna were fed concentrated freshwater suspensions of unicellular green algae Desmodesmus Subspicatus and Pseudokirchneriella subcapitata at a rate equivalent to 0.1-0.2mg of organic carbon per adult per day.
ACCLIMATION
- Type and amount of food: concentrated freshwater suspensions of unicellular green algae Desmodesmus Subspicatus and Pseudokirchnerie/la subcapitata five times per week at a rate equivalent to 0.20mg of organic carbon per adult per day.
- Feeding frequency: daily - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
- Remarks on exposure duration:
- N/A
- Post exposure observation period:
- none
- Hardness:
- 165-175 mg/L CaCO3
- Test temperature:
- 20 - 20.8 °C
- pH:
- 7.79-8.35.
- Dissolved oxygen:
- 8.81-9.44 mg/L
- Salinity:
- according to guideline (M4 medium, Elendt)
- Conductivity:
- N/A
- Nominal and measured concentrations:
- Nominal: 100 mg/L (saturated solution of the test substance)
Measured: < LOD (0.214 mg/L) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): illumination incubabor with 75 ml glass containers
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): 3 times per week (on the days 0, 2, 5, 7, 9, 12,1 4, 16, 19, 21 d)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: not mentioned
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water according to Elendt M4-Medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Day 0 (fresh samples), 2, 5, 7, 12, 14 d
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light, 8h dark
- Light intensity: not exceeding 1125-1326 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and offspring on day 2, 5, 7, 9, 12, 14, 16, 19, 21
TEST CONCENTRATIONS
- Range finding study:
The test substance is insoluble. 2 L solution of the test substance, the nominal concentration of which was 100 mg/L, was prepared with Elendt M4 medium and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm and were filtrated with 47 mm glass microfiber filters and 0.45 µm cellulose nitrate membrane filters to make a saturated solution of the test substance in the test medium as the stock solution and the test solutions of the highest concentration group. The test solutions were prepared once every week and subpackage stored at room temperature for 7 days. The pH of the stock solution measured before the test was 7.77 and needed not to be adjusted. Three treatment groups and one test-medium control series were included in the range-finding test. The nominal concentrations of the treatment group were 1%, 10% and 100% saturation solution. 5 animals individually held were tested for each test concentration. Each test concentration series consisted of replicates filled with test solutions of the same concentration. One animal was maintained individually in each replicate. The duration of the range-finding test was 21 days. The test vessels were not aerated during the test. The range-finding test was performed in the illumination incubabor with 75 mL glass containers and the ratio of light to dark was 16 h to 8 h. Parent animals were maintained individually, one per test vessel, with about 75mL of medium in each vessel.
A semi-static test was conducted. The frequency of medium renewal was three times per week in the range-finding test. The medium was renewed on the days 0, 2, 5, 7, 9, 12, 14, 16, 19d of the test. Every adult was fed concentrated freshwater suspensions of unicellular green algae Desmodesmus Subspicatus and Pseudokirchneriella subcapitata five times per week at a rate equivalent to 0.20mg of organic carbon per adult per day. The results of the observations made during the test were recorded five times per week. The offspring produced by each parent animal were removed and counted from the appearance of the first brood. The numbers of living offspring, aborted eggs and dead offspring were counted and recorded. Mortality among the parent animals was recorded at the same times as offsprings were counted. During the test, when the medium was renewed in the semi-static test, a second series of test vessels had been prepared and the parent animals were transferred to them by a glass pipette of suitable diameter.
The mortality of the parent animals in the control group was 0%. The mortality of the parent animals in the treatment groups was varied from 0% to 20%. According to the result of the Steel's Many-One Rank Test, the 21d NOEC for cumulative adult deaths of the test substance to Daphnia magna was the concentration of the 100% saturated solution in the test medium and the LOEC was higher than the concentration of the 100% saturated solution in the test medium. The death of the parent animals in all treatment groups was not related to the effect of the test substance. According to the result of the Dunnett's Test, the 21d NOEC for reproduction of the test substance to Daphnia magna was the concentration of the 100% saturated solution in the test medium and the LOEC was higher than the concentration of the 100% saturated solution in the test medium. Based on the result of range-finding test, a limit test was carried out. - Reference substance (positive control):
- no
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- N/A
- Results with reference substance (positive control):
- N/A
- Reported statistics and error estimates:
- Dunnett's Test.
Statistical analysis of the data was accomplished with Toxcalc v5.0.32. - Validity criteria fulfilled:
- yes
- Conclusions:
- Expressed as the concentration of the 100% saturated solution in the test medium, the effect of 1,2,4-BENZENETRICARBOXYLIC ACID, MIXED DODECYL AND OCTYL TRIESTERS on Daphnia magna reproductive output is as following: The 21d No Observed Effect Concentration (NOEC) was not less than the concentration of the saturation solution in the test medium (the measured concentration of the 100% saturated solution was below the limit of detection (LOD) and the LOD was 0.214mg/L); The 21d Lowest Observed Effect Concentration (LOEC) was higher than the concentration of the saturation solution in the test medium (the measured concentration of the 100 % saturated solution was below the limit of detection (LOD) and the LOD was 0.214 mg/L); The 21d EC50 (50% reduction of the reproductive output) was higher than the concentration of the saturation solution in the test medium (the measured concentration of the 100% saturated solution was below the limit of detection (LOD) and the LOD was 0.214mg/L). The mortality of the parent animals in control group was 0%, not exceed 20%, and the mortality of the parent animals in the treatment group was 0% at the end of the test. No toxicity was observed under test conditions in the range of water solubility.
- Executive summary:
According to "Ministry of environmental protection of the people's Republic of China, The Guidelines for the Testing of Chemicals - Effects an Biotic Systems, 2013, 211 Daphnia magna Reproduction Test" and "OECD, Guidelines for the Testing of Chemicals, 211 Daphnia magna Reproduction Test, 2012", the effects of the test substance 1,2,4-BENZENETRICARBOXYLIC ACID, MIXED DODECYL AND OCTYL TRIESTERS to Daphnia magna was determined in a 21 days reproduction test. The frequency of medium renewal was three times per week. Based an the results of the range-finding test, one treatment group and a control group were included in the limit test. A nominal concentration of 100 mg/L of the test substance was prepared and stirred (via magnetic stirrer) in the dark for 72h at 500 rpm followed by filtration using a 47 mm glass microfiber filters and 0.45 µm cellulose nitrate membrane filters to make a saturated solution of the test substance in the test medium as the test solution (referred to as "100 % saturated solution" in this report) of the treatment group. The test solutions were prepared once every week and stored at room temperature for 7 days.
The mortality of the parent animals in control group was 0%, not exceed 20%, and the mortality of the parent animals in the treatment group was 0 % at the end of the test. The mean number of live offspring produced per parent animal surviving at the end of the test in control group was 87, not less than 60, and the coefficient of variation of which was 12 %, not exceed 25 %.The test is valid.
The concentrations of the test substance in the test solutions were analyzed by using GC method. The measured concentration of the 100% saturated solution was below the limit of detection (LOD) in the preliminary test and the LOD was 0.214mg/L. Therefore the results were expressed as the concentration of the 100 % saturated solution.
Results showed that under the testing conditions, for Daphnia magna reproductions, the NOEC was not less than the concentration of the 100% saturated solution in the test medium, the LOEC was higher than the concentration of the 100% saturated solution in the test medium, and 50% reduction in reproductive output (EC50) was higher than the concentration of the saturated solution in the test medium. No toxicity was observed under test conditions in the range of water solubility.
The measured concentration of the 100% saturated solution was below the limit of detection (LOD) and the LOD was 0.214 mg/L.
Reference
Table #1: Number of live offspring and the coefficient of variation for control fecundity
Nominal concentration groups of test substance |
Day | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
The control | 8 | 5 | 0 | 4 | 4 | 1 | 0 | 3 | 1 | 3 | 7 |
9 | 7 | 6 | 9 | 11 | 3 | 4 | 7 | 0 | 6 | 10 | |
12 | 17 | 14 | 16 | 19 | 10 | 16 | 17 | 7 | 13 | 20 | |
13 | 20 | 21 | 8 | 19 | 19 | 14 | 23 | 17 | 26 | 19 | |
14 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | |
15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
16 | 17 | 19 | 15 | 17 | 16 | 13 | 12 | 15 | 15 | 14 | |
19 | 34 | 28 | 28 | 28 | 31 | 24 | 29 | 33 | 26 | 29 | |
20 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | |
21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Total | 100 | 88 | 80 | 98 | 80 | 72 | 92 | 74 | 89 | 99 | |
Mean | 87 | ||||||||||
CV (%) | 12 % | ||||||||||
The 100 % saturated solution | 9 | 3 | 0 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
12 | 11 | 16 | 13 | 10 | 13 | 10 | 13 | 11 | 9 | 11 | |
13 | 15 | 25 | 18 | 0 | 21 | 18 | 18 | 16 | 12 | 18 | |
14 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 1 | 0 | |
15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
16 | 18 | 20 | 12 | 0 | 19 | 14 | 18 | 15 | 0 | 17 | |
19 | 30 | 32 | 31 | 0 | 31 | 26 | 30 | 26 | 4 | 26 | |
20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
21 | 0 | 27 | 38 | 0 | 30 | 26 | 32 | 32 | 0 | 31 | |
Total | 77 | 120 | 116 | 14 | 115 | 94 | 114 | 103 | 26 | 103 | |
Mean | 88 | ||||||||||
CV (%) | 43 % |
Description of key information
The effect of 1,2,4-Benzenetricarboxylic acid, mixed dodecyl and octyl triesters on Daphnia magna reproductive output, expressed as The concentration of the 100% saturated solution in the test medium,is as following:
The 21d NOEC was not less than the concentration of the saturation solution in the test medium (the measured concentration of the 100% saturated solution was below the limit of detection (LOD) and the LOD was 0.214mg/L). No toxicity was observed under test conditions in the range of water solubility.;
The 21d LOEC was higher than the concentration of the saturation solution in the test medium (the measured concentration of the 100 % saturated solution was below the limit of detection (LOD) and the LOD was 0.214 mg/L);
The 21d EC50 (50% reduction of the reproductive output) was higher than the concentration of the saturation solution in the test medium (the measured concentration of the 100% saturated solution was below the limit of detection (LOD) and the LOD was 0.214mg/L).
The mortality of the parent animals in control group was 0%, not exceed 20%, and the mortality of the parent animals in the treatment group was 0% at the end of the test.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- >= 100 mg/L
Additional information
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