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EC number: 700-342-7 | CAS number: 1163775-81-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-04-19 - 2014-12-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- N/A
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: For the analysis of the actual test substance concentrations, 30 mL of test solution was taken from each test vessel at the beginning and at the end of the test.
- Sample storage conditions before analysis: directly analyzed without storage - Vehicle:
- no
- Details on test solutions:
- 500.8 mg test substance was added in 5 L test water and a nominal concentration of 100 mg/Lof the test substance was prepared and stirred (via magnetic stirrer) in the dark at 500 rpm followed by filtration using a 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water.
- Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: Minnow
- Strain: Gobiocypris rarus
- Source: Bioassay and Safety Assessment Lab, Shanghai Academy of Public Measurement
- Age at study initiation (mean and range, SD): not mentioned
- Feeding during test: none
ACCLIMATION
- Acclimation period: more than 14 days
- Acclimation conditions (same as test or not): The test water used was tapwater dechlorinated by activated carbon and sterilyzed by UV lamp.
Temperature 21-25 °C and constant with a range of 2 °C. Oxygen concentration: > 80 % of air saturation value (ASV), Photoperiod: Light : Dark = 12 hours :12 hours.
- Length was 2.2 ± 0.1 cm (Mean ± SD) and weight was 0.18 ± 0.03 g (Mean ± SD) at the end of the test.
- Feeding frequency: daily until 24 hours before the test was started
- Health during acclimation (any mortality observed): No diseases were observed during the acclimation period. No mortality was present the 7 days before the start of the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 145 mg CaCO3/L
- Test temperature:
- 23.8 - 24.3 °C
- pH:
- 6 - 8.5
- Dissolved oxygen:
- 75.3 - 91.8 %
- Salinity:
- N/A
- Conductivity:
- N/A
- Nominal and measured concentrations:
- Nominal: 100 mg/L WAF
Measured: below LOD of 0.274 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 5 L glass tanks containing 4 L test solution.
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.5 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, dechlorinated by activated carbon and sterilized by UV lamp. The water was aerated continously over 24 h.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 hours light, 12 hours darkness
- Light intensity: not mentioned
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 24, 48, 72 and 96 hours; pH, temperature, dissolved Oxygen at the same time.
At the end of test, the body length and body weight of the test fish of the control were measured.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test, test concentration limited by test item's water solubility)
- Range finding study: The log Pow of the test substance was estimated to 13.4, it is considered to have an adsorption. Therefore, the test vessels and membranes were pretreated (supersaturated) before the test. 501.3 mg test substance was prepared and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm followed by filtration using a 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water. One concentration of the saturated solution (the measured concentration was below the LOD of 0.274 mg/L) was used. In this test, a blank control of test water was used. Five fishes were observed and dead fish were recorded at 0, 24, 48, 72 and 96 h after test initiation. In the range-finding test, there were no mortalities and abnormalities observed in the fish of the control group and the treatment group during the test. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: no mortality at limit of water solubility
- Details on results:
- N/A
- Results with reference substance (positive control):
- no reference substance tested
- Reported statistics and error estimates:
- no statisitcs performed, as no mortality occured
- Sublethal observations / clinical signs:
The mortatlities and abnormalities in limit test (10 fish in each concentration):
Group Duration (h) Mortalities Cumulative Mortalities Abnormalities Blank control 0 0 0 none 24 0 0 none 48 0 0 none 72 0 0 none 96 0 0 none Treatment Group (Saturated solution) 0 0 0 none 24 0 0 none 48 0 0 none 72 0 0 none 96 0 0 none - Validity criteria fulfilled:
- yes
- Conclusions:
- There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution under the test conditions. Not toxic under test conditions in the range of water solubility.
- Executive summary:
According to "Ministry of environmental protection of the people's Republic of China, the guidelines for the testing of chemicals-effects on biotic system, 2013, 203 Fish, OECD 203, the acute toxicity of the test substance to Gobiocypris rarus was determined by a static test. A nominal concentration of 100 mg/L of the test substance was prepared and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm followed by filtration using 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water (referred to as "saturated solution" in this report). The octanol/water partition coefficient (log Pow) of the test substance was estimated to 13.4, it is considered to have an adsorption. Therefore, the test vessels and membranes were pretreated (supersaturated) before the test. Based on the results of the range-finding test, the saturated solution was used to perform the limit test with a blank control. The test duration was 96h. No mortality occurred in the control during the test and the dissolved oxygen concentration was greater than 60 % at the air saturation value throughout the test duration. Hence, the test was considered to be valid.
During the test, the measured concentrations of the test substance analyzed by Gas Chromatograph (GC) were below the limit of detection (LOD, LOD = 0.274 mg/L). There were no mortalities and abnormalities observed in the treatment group during the test period. There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution (which measured concentration was below LOD, LOD = 0.274 mg/L) under the test conditions.
Reference
Description of key information
There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution under the test conditions. Not toxic under test conditions in the range of water solubility.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 100 mg/L
Additional information
According to "Ministry of environmental protection of the people's Republic of China, the guidelines for the testing of chemicals-effects on biotic system, 2013, 203 Fish, OECD 203, the acute toxicity of the test substance to Gobiocypris rarus was determined by a static test. A nominal concentration of 100 mg/L of the test substance was prepared and stirred (via magnetic stirrer) in the dark for 72 h at 500 rpm followed by filtration using 0.45 µm nitrocellulose membrane to make a saturated solution of the test substance in the test water (referred to as "saturated solution" in this report). The octanol/water partition coefficient (log POW) of the test substance was estimated to 13.4, it is considered to have an adsorption. Therefore, the test vessels and membranes were pretreated (supersaturated) before the test. Based on the results of the range-finding test, the saturated solution was used to perform the limit test with a blank control. The test duration was 96h. No mortality occured in the control during the test and the dissolved oxygen concentration was greater than 60 % at the air saturation value throughout the test duration. Hence, the test was considered to be valid.
During the test, the measured concentrations of the test substance analyzed by Gas Chromatograph (GC) were below the limit of detection (LOD, LOD = 0.274 mg/L). There were no mortalities and abnormalities observed in the treatment group during the test period. There was no acute toxicity of the saturated solution in the limit test. The test results showed that the 96 h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution (which measured concentration was below LOD, LOD = 0.274 mg/L) under the test conditions.
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